Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, S.H.; Cao, M.J.; Huang, J.Z.; Wu, G.P.
    Identification of a puromycin-sensitive aminopeptidase from zebrafish (Danio rerio) (2011), Comp. Biochem. Physiol. B, 159, 10-17.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.14 1,10-phenanthroline 19.4% residual activity at 0.2 mM Danio rerio
3.4.11.14 bestatin 1.1% residual activity at 0.05 mM Danio rerio
3.4.11.14 CdCl2 6.2% residual activity at 1 mM Danio rerio
3.4.11.14 CuSO4 0.6% residual activity at 1 mM Danio rerio
3.4.11.14 E-64 27.2% residual activity at 0.015 mM Danio rerio
3.4.11.14 EDTA 17.5% residual activity at 5 mM, 3,3% residual activity after extensively dialysis against 1 mM, restored by 0.01 mM Zn2+ to 57.8% activity Danio rerio
3.4.11.14 EGTA 24.3% residual activity at 5 mM Danio rerio
3.4.11.14 L-arginine 19.3% residual activity at 5 mM Danio rerio
3.4.11.14 L-cysteine non-competitive, 7.3% residual activity at 5 mM Danio rerio
3.4.11.14 L-isoleucine 20.1% residual activity at 5 mM Danio rerio
3.4.11.14 L-leucine 6.9% residual activity at 5 mM Danio rerio
3.4.11.14 L-phenylalanine 21.1% residual activity at 5 mM Danio rerio
3.4.11.14 L-tryptophan 15.5% residual activity at 5 mM Danio rerio
3.4.11.14 L-valine 23.7% residual activity at 5 mM Danio rerio
3.4.11.14 puromycin 9.5% residual activity at 0.01 mM Danio rerio
3.4.11.14 ZnSO4 24% residual activity at 1 mM Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.14 0.0013
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.0019
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.0033
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.0052
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.0066
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.01
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.035
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.045
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 0.24
-
 glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.14 98000
-
 gel filtration Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.14 Danio rerio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.14 ion exchange chromatography (DEAE), hydroxyapatite chromatography, ammonium sulfate precipitation, hydrophobic interaction chromatography Danio rerio

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.14 glycine-4-methyl-coumaryl-7-amide + H2O
-
 Danio rerio glycine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-arginine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-lysine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-lysine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-methionine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-methionine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-proline-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-proline + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-tyrosine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-tyrosine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.14 L-valine-4-methylcoumaryl-7-amide + H2O
-
 Danio rerio L-valine + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.14 monomer 98000, SDS-PAGE, gel filtration Danio rerio

Synonyms

EC Number Synonyms Comment Organism
3.4.11.14 puromycin-sensitive aminopeptidase
-
 Danio rerio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.14 45
-
-
 Danio rerio

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.14
-
 45 unstable above 50°C Danio rerio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.14 0.9
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 1.1
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 3.8
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 8.2
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 10.3
-
 glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 17.1
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 25.6
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 38.5
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 48.9
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.14 7.5
-
-
 Danio rerio

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.14 7
-
 activity decreases rapidly when pH value higher or lower than 7.0 Danio rerio

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.14 0.27
-
 L-cysteine pH 7.0, 37°C Danio rerio

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.14 40
-
 glycine-4-methyl-coumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 170
-
 L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 260
-
 L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 870
-
 L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 1400
-
 L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 2500
-
 L-methionine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 3000
-
 L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 3300
-
 L-lysine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio
3.4.11.14 4200
-
 L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Danio rerio