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Literature summary extracted from

  • Dellas, N.; Noel, J.P.
    Mutation of archaeal isopentenyl phosphate kinase highlights mechanism and guides phosphorylation of additional isoprenoid monophosphates (2010), Chem. Biol., 5, 589-601.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.26 X-ray structure determination Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.26 F83A/I86A/I156A the mutant shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. The reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii
2.7.4.26 H60Q His60 plays a key role in binding and catalysis, the mutant enzyme shows increased values for Km and decreased vaules for kcat and kcat/Km Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.26 0.0043
-
 isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
2.7.4.26 0.0345
-
 isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
2.7.4.26 0.198
-
 ATP wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
2.7.4.26 0.559
-
 ATP mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.26 ATP + isopentenyl phosphate Methanocaldococcus jannaschii
-
 ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate Methanocaldococcus jannaschii DSM 2661
-
 ADP + isopentenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.26 Methanocaldococcus jannaschii Q60352
-
-
2.7.4.26 Methanocaldococcus jannaschii DSM 2661 Q60352
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.26 ATP + (2E,6E)-farnesyl phosphate the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii ADP + (2E,6E)-farnesyl diphosphate
-
 ?
2.7.4.26 ATP + (2E,6E)-farnesyl phosphate the mutant F83A/I86A/I156A shows a 27% rate of conversion of (2E,6E)-farnesyl phosphate to (2E,6E)-farnesyl diphosphate. This reaction is not catalyzed by the wild-type enzyme Methanocaldococcus jannaschii DSM 2661 ADP + (2E,6E)-farnesyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanocaldococcus jannaschii ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Methanocaldococcus jannaschii DSM 2661 ADP + isopentenyl diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.26 0.04
-
 isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
2.7.4.26 1.46
-
 isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.26 1
-
 isopentenyl phosphate mutant H60Q, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii
2.7.4.26 340
-
 isopentenyl phosphate wild-type enzyme, pH 8.0, temperature not specified in the publication Methanocaldococcus jannaschii