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Literature summary extracted from

  • Chau, M.; Jennewein, S.; Walker, K.; Croteau, R.
    Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7beta-hydroxylase (2004), Chem. Biol., 11, 663-672.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.182 expressed in a Spodoptera fugiperda-baculovirus-based expression system Taxus cuspidata

General Stability

EC Number General Stability Organism
1.14.14.182 taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7 Taxus cuspidata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.182 0.0076
-
 (+)-taxusin pH 7.5, temperature not specified in the publication Taxus cuspidata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.182 microsome
-
 Taxus cuspidata
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.182 Taxus cuspidata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.182 P-93AF cell
-
 Taxus cuspidata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.182 (+)-taxusin + O2 + NADPH + H+ i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate Taxus cuspidata 7beta-hydroxytaxusin + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.182 T7H
-
 Taxus cuspidata
1.14.14.182 taxoid 7beta-hydroxylase
-
 Taxus cuspidata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.182 7.5
-
-
 Taxus cuspidata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.182 cytochrome P450
-
 Taxus cuspidata
1.14.14.182 NADPH
-
 Taxus cuspidata