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Literature summary extracted from
- Stability profiles of nepenthesin in urea and guanidine hydrochloride: comparison with porcine pepsin A (2010), Biosci. Biotechnol. Biochem., 74, 2323-2326.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.1 | dithiothreitol | - |
Sus scrofa |  |
| 3.4.23.12 | dithiothreitol | isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol | Nepenthes distillatoria | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.4.23.1 | guanidine-HCl | pepsin A is stable up to 3.6 M and inactivated at 5 M guanidine-HCl | Sus scrofa |
| 3.4.23.1 | urea | pepsin A is stable up to at least 5.6 M urea | Sus scrofa |
| 3.4.23.12 | dithiothreitol | isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol | Nepenthes alata |
| 3.4.23.12 | guanidine-HCl | completely inactivated in the presence of 3 M, the midpoints of denaturation are 4.3 M, 0.4 M and 1.2 M guanidine-HCl for isoform nethepsin I, IIa and IIb, respectively | Nepenthes alata |
| 3.4.23.12 | guanidine-HCl | completely inactivated in the presence of 3 M, the midpoints of denaturation are 4.3 M, 0.4 M and 1.2 M guanidine-HCl for isoform nethepsin I, IIa and IIb, respectively | Nepenthes distillatoria |
| 3.4.23.12 | urea | activity is completely lost in the presence of 5 M urea, the midpoints of denaturation are 1.7 M, 2.4 M and 1.6 M urea for isoform nethepsin I, IIa and IIb, respectively | Nepenthes alata |
| 3.4.23.12 | urea | activity is completely lost in the presence of 5 M urea, the midpoints of denaturation are 1.7 M, 2.4 M and 1.6 M urea for isoform nethepsin I, IIa and IIb, respectively | Nepenthes distillatoria |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.1 | Sus scrofa | - |
- |
- |
| 3.4.23.12 | Nepenthes alata | - |
- |
- |
| 3.4.23.12 | Nepenthes distillatoria | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.12 | DEAE-cellulose column chromatography, pepstatin column chromatography, MonoQ column chromatography, and Sephacryl S-200 gel filtration | Nepenthes alata |
| 3.4.23.12 | DEAE-cellulose column chromatography, pepstatin column chromatography, MonoQ column chromatography, and Sephacryl S-200 gel filtration | Nepenthes distillatoria |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.12 | pitcher secretion | - |
Nepenthes alata | - |
| 3.4.23.12 | pitcher secretion | - |
Nepenthes distillatoria | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.12 | Hemoglobin + H2O | - |
Nepenthes alata | ? | - |
? | |
| 3.4.23.12 | Hemoglobin + H2O | - |
Nepenthes distillatoria | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.12 | nepenthesin I | isoform | Nepenthes alata |
| 3.4.23.12 | nepenthesin I | isoform | Nepenthes distillatoria |
| 3.4.23.12 | nepenthesin IIa | isoform | Nepenthes alata |
| 3.4.23.12 | nepenthesin IIa | isoform | Nepenthes distillatoria |
| 3.4.23.12 | nepenthesin IIb | isoform | Nepenthes alata |
| 3.4.23.12 | nepenthesin IIb | isoform | Nepenthes distillatoria |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 50 | - |
pepsin A is completely inactivated at pH 3.0 and 50°C after 7 days | Sus scrofa |
| 3.4.23.12 | 53 | - |
stable up to 53°C during 1 h of incubation | Nepenthes alata |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.1 | 3 | 10 | pepsin A is completely inactivated at pH 3.0-10.0 at 37°C after 30 days, the enzyme is unstable at pH 7.0 and above (irreversible inhibition) | Sus scrofa |
| 3.4.23.12 | 2.9 | - |
at pH 2.9 at 37°C, activity decreased very slowly on longer incubation, about 60% and 30% of the original activity are retained after 30 days and 65 days, respectively | Nepenthes alata |
| 3.4.23.12 | 3 | 10 | when nepenthesin I is incubated at pH 3.0 at 60°C for 30 days, about 30% of the original activity remains. When incubated at pH 3.0-10.0 at 37°C for 30 days, the enzyme retains 80% or more of activity | Nepenthes distillatoria |
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