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Literature summary extracted from

  • Raevskaya, A.A.; Kuznetsova, E.M.; Savvateeva, M.V.; Severin, S.E.
    Isolation, purification, and study of properties of recombinant hepsin from Escherichia coli (2010), Biochemistry, 75, 866-872.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.106 resveratrol hepsin activity increases in dose-dependent manner in the presence of resveratrol Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.106 expressed in Escherichia coli strains BL21(DE3), BL21(DE3) Codon Plus RIL, and Rosetta pLysS Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.106 anthralin at 0.067 mM anthralin the hepsin activity is reduced by more than 70% Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.106 0.05
-
 L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide in 0.3M Tris-HCl, 0.3 M imidazole, and 0.5 M NaCl, pH 8.4, at 37°C Homo sapiens
3.4.21.106 0.05
-
 L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide in 0.3M Tris-HCl, 0.3 M imidazole, and 0.5 M NaCl, pH 8.4, at 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.106 42038
-
 x * 42038, calculated from amino acid sequence Homo sapiens
3.4.21.106 43000
-
 x * 43000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.106 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.106 Ni-IDA.Sepharose column chromatography and Q-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.106 L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-Asp-L-Ala-L-Ala-L-Arg + 4-nitroaniline
-
 ?
3.4.21.106 L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Homo sapiens L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.106 ? x * 43000, SDS-PAGE Homo sapiens
3.4.21.106 ? x * 42038, calculated from amino acid sequence Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.106 Homo sapiens calculated from amino acid sequence
-
 7.3