EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.18.1.5 | crystal structure of a covalently linked putidaredoxin reductase (Pdr)-putidaredoxin (Pdx) complex. Residues R65 and R310 are the key elements required for the formation of a productive electron transfer complex with Pdx. The C-terminal lysine cluster assists in Pdx docking by fine-tuning Pdr-Pdx interactions to achieve the optimal geometry between the redox centers, and the basic surface residues in Pdr-like ferredoxin reductases not only define specificity for the redox partner but also may facilitate its dissociation | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.18.1.5 | K339A | moderate decrease in the binding affinity and reduction of Pdx | Pseudomonas putida |
1.18.1.5 | K387A | moderate decrease in the binding affinity and reduction of Pdx | Pseudomonas putida |
1.18.1.5 | K409A | moderate decrease in the binding affinity and reduction of Pdx | Pseudomonas putida |
1.18.1.5 | N384A | mutation has no effect on the Pdr-Pdx interaction | Pseudomonas putida |
1.18.1.5 | R310A | mutation lowers the interprotein electron tranfer rate by 20-30fold without perturbing the Pdx association step | Pseudomonas putida |
1.18.1.5 | R310E | mutation decreases both the Pdr-to-Pdx ET and partner binding affinity by 100- and 8fold, respectively | Pseudomonas putida |
1.18.1.5 | R65A | mutation lowers the interprotein electron tranfer rate by 20-30fold without perturbing the Pdx association step | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.5 | 0.017 | - |
NADH | mutant R65A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.018 | - |
NADH | mutant R310A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.022 | - |
NADH | mutant R339A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.025 | - |
NADH | mutant R339A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.027 | - |
NADH | wild-type, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.029 | - |
NADH | mutant K409A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.029 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R65A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.031 | - |
NADH | mutant K409A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.034 | - |
NADH | mutant R65A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.036 | - |
NADH | mutant K387A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.036 | - |
Fe(CN)63- | mutant K409A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.036 | - |
NADH | mutant R310A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.037 | - |
NADH | mutant R310E, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.037 | - |
NADH | mutant R310E, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.038 | - |
NADH | wild-type, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.04 | - |
Fe(CN)63- | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.04 | - |
Fe(CN)63- | mutant R310E, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.042 | - |
NADH | mutant K387A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.043 | - |
Fe(CN)63- | mutant N384A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.044 | - |
Fe(CN)63- | mutant K387A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.045 | - |
Fe(CN)63- | mutant R339A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.05 | - |
NADH | mutant N384A, pH 7.5, 25°C, cosubstrate dichlorophenolindophenol | Pseudomonas putida | |
1.18.1.5 | 0.05 | - |
NADH | mutant N384A, pH 7.5, 25°C, cosubstrate K3Fe(CN)6 | Pseudomonas putida | |
1.18.1.5 | 0.05 | - |
Fe(CN)63- | mutant R65A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.083 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant K387A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.083 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant N384A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.091 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant K409A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.097 | - |
oxidized 2,6-dichlorophenolindolphenol | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.125 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R310A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.125 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R339A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.143 | - |
Fe(CN)63- | mutant R310A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 0.2 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R310E, pH 7.5, 25°C | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.5 | Pseudomonas putida | P16640 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.5 | 2 ferricytochrome c + NADH | - |
Pseudomonas putida | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
1.18.1.5 | NADH + H+ + Fe(CN)63- | - |
Pseudomonas putida | NAD+ + Fe(CN)62- | - |
? | |
1.18.1.5 | NADH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Pseudomonas putida | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.18.1.5 | reduced putidaredoxin + NAD+ | - |
Pseudomonas putida | oxidized putidaredoxin + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.5 | Pdr | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.5 | 203 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R65A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 283 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R310E, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 288 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R339A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 358 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant N384A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 377 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant K409A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 385 | - |
oxidized 2,6-dichlorophenolindolphenol | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 408 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant K387A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 543 | - |
Fe(CN)63- | mutant R339A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 560 | - |
Fe(CN)63- | mutant R65A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 617 | - |
oxidized 2,6-dichlorophenolindolphenol | mutant R310A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 650 | - |
NADH | mutant R339A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 720 | - |
NADH | mutant R310E, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 729 | - |
NADH | mutant R310A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 754 | - |
NADH | mutant R65A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 755 | - |
Fe(CN)63- | mutant K409A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 772 | - |
Fe(CN)63- | mutant K387A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 780 | - |
NADH | mutant K387A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 790 | - |
Fe(CN)63- | mutant R310E, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 800 | - |
NADH | mutant K409A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 806 | - |
NADH | wild-type, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 820 | - |
NADH | mutant N384A, pH 7.5, 4°C | Pseudomonas putida | |
1.18.1.5 | 898 | - |
Fe(CN)63- | mutant N384A, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 982 | - |
Fe(CN)63- | wild-type, pH 7.5, 25°C | Pseudomonas putida | |
1.18.1.5 | 1485 | - |
Fe(CN)63- | mutant R310A, pH 7.5, 25°C | Pseudomonas putida |