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Literature summary extracted from

  • Mikhailova, A.G.; Khairullin, R.F.; Demidyuk, I.V.; Gromova, T.Y.; Kostrov, S.V.; Rumsh, L.D.
    Oligopeptidase B from Serratia proteamaculans. II. Enzymatic characteristics: substrate analysis, influence of calcium ions, pH and temperature dependences (2011), Biochemistry (Moscow), 76, 480-490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide
-
 Serratia proteamaculans
3.4.21.83 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide
-
 Serratia proteamaculans
3.4.21.83 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide
-
 Serratia proteamaculans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.0226
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.0513
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.058
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.084
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.09
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.096
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.224
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.265
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.302
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.306
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.322
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.457
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.54
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 1.05
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 2.2
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.83 Ca2+ in presence of Ca2+, efficiency of hydrolysis of substrates with positively charged P2 residues decreases by an order of magnitude due to the worsening in the substrate binding. In the presence of 50 mM Ca2+, no substrate inhibition is observed in the case of acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide, and the effect was less pronounced for other substrates Serratia proteamaculans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 78000
-
 x * 78000, SDS-PAGE Serratia proteamaculans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.83 dimethyl sulfoxide assay in presence of 2% dimethyl sulfoxide Serratia proteamaculans

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Serratia proteamaculans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans acetyl-L-Leu-L-Leu-L-Arg + 4-nitroaniline
-
 ?
3.4.21.83 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline
-
 ?
3.4.21.83 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
-
 ?
3.4.21.83 Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
-
 Serratia proteamaculans Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?
3.4.21.83 Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O poor substrate Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-Ala-L-Ala-L-Arg + 4-nitroaniline
-
 ?
3.4.21.83 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester + H2O
-
 Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-lysine + phenylmethanethiol
-
 ?
3.4.21.83 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans Nalpha-benzyloxycarbonyl-L-Phe-L-Arg + 4-nitroaniline
-
 ?
3.4.21.83 succinyl-Gly-L-Pro-L-Lys-4-nitroanilide + H2O poor substrate Serratia proteamaculans succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline
-
 ?
3.4.21.83 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide + H2O
-
 Serratia proteamaculans succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 4-nitroaniline
-
 ?
3.4.21.83 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide + H2O
-
 Serratia proteamaculans succinyl-L-Ala-L-Ala-L-Pro-L-Arg + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.83 ? x * 78000, SDS-PAGE Serratia proteamaculans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.83 25
-
-
 Serratia proteamaculans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 30
-
 rapid loss of activity above Serratia proteamaculans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.83 0.5
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 1
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 1.2
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 2 8 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 7.6
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 13.9
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 14.5
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 21.2
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 24.3
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 32
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 35.2
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 37.7
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 53.8
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 55.5
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 201.7
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.83 8.5
-
-
 Serratia proteamaculans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.83 7.5 9.5
-
 Serratia proteamaculans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.83 0.027
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.045
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.126
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.19
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.4
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.83 0.07
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.4
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Lys-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 0.5
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 2
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 3
-
 succinyl-L-AlaL-Ala-L-Pro-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 5.8
-
 Nalpha-benzyloxycarbonyl-L-lysine thiobenzyl ester pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 10.8
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 30
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 34
-
 acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 120
-
 acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 125
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 161
-
 Nalpha-benzoyl-DL-arginine-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 251
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 377
-
 benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 485
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide pH 8.0, 25°C Serratia proteamaculans
3.4.21.83 680
-
 Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide presence of 50 mM Ca2+, pH 8.0, 25°C Serratia proteamaculans