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Literature summary extracted from

  • Rudakova, N.L.; Balaban, N.P.; Danilova, Y.V.; Rudenskaya, G.N.; Sharipova, M.R.
    Characteristics of a novel secreted zinc-dependent endopeptidase of Bacillus intermedius (2010), Biochemistry (Moscow), 75, 1294-1301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.28 gene mprBi, DNA and amino acid sequence determination and analysis, enzyme expression in Bacillus subtilis and secretion to the culture medium Bacillus intermedius

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.28 1,10-phenanthroline 94.2% inhibition at 0.5 mM, 100% at 5 mM Bacillus intermedius
3.4.24.28 Co2+
-
 Bacillus intermedius
3.4.24.28 Cu2+
-
 Bacillus intermedius
3.4.24.28 EDTA 4% inhibition at 0.5 mM, 94.3% at 5 mM Bacillus intermedius
3.4.24.28 HgCl2 49% inhibition at 0.5 mM, 60% at 5 mM Bacillus intermedius
3.4.24.28 additional information no inhibition by protein trypsin inhibitor Bacillus intermedius
3.4.24.28 Ni2+
-
 Bacillus intermedius
3.4.24.28 PCMB 6% inhibition at 0.5 mM, 98.1% at 5 mM Bacillus intermedius
3.4.24.28 PMSF 6% inhibition at 0.5 mM, 9% at 5 mM Bacillus intermedius
3.4.24.28 Zn2+ secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme Bacillus intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.28 0.06
-
 azocasein recombinant wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.28 extracellular secreted zinc-dependent endopeptidase Bacillus intermedius
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.28 Ca2+ 30% activation at 10 mM Bacillus intermedius
3.4.24.28 Mg2+ 20% activation at 10 mM Bacillus intermedius
3.4.24.28 Zn2+ secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme Bacillus intermedius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.28 19000
-
 x * 19000, SDS-PAGE Bacillus intermedius

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.28 Bacillus intermedius B3V4Z0 gene mprBi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.28 recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity Bacillus intermedius

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.28 0.0057
-
 with substrate Dnp-AAVR, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 0.0145
-
 with substrate Dnp-GGLR, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 0.0264
-
 with substrate Dnp-GGK, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 0.0282
-
 with substrate Dnp-GGIR, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 0.0365
-
 with substrate Dnp-GGFR, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 0.0515
-
 with substrate Dnp-AALR-NH2, pH 8.0, 37°C Bacillus intermedius
3.4.24.28 17.4
-
 with substrate azocasein, recombinant purified wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.28 azocasein + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-AALR-NH2 + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-AAVR + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-GGFR + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-GGIR + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-GGK + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 Dnp-GGLR + H2O
-
 Bacillus intermedius ?
-
 ?
3.4.24.28 additional information analysis of substrate specificity of MprBi using synthetic chromogenic substrates, MALDI-TOF mass spectrometric product analysis, overview Bacillus intermedius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.28 ? x * 19000, SDS-PAGE Bacillus intermedius

Synonyms

EC Number Synonyms Comment Organism
3.4.24.28 MprBi
-
 Bacillus intermedius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.28 37
-
 assay at Bacillus intermedius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.28 22 70 activity range Bacillus intermedius

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.28 1210
-
 azocasein recombinant wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.28 8
-
 asay at Bacillus intermedius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.28 Bacillus intermedius
-
-
 5.4

General Information

EC Number General Information Comment Organism
3.4.24.28 evolution MprBi is a metzincin clan adamalysin/reprolysin-like metalloprotease and belongs to the class of zinc-dependent metalloproteinases Bacillus intermedius