BRENDA - Enzyme Database

Purification and characterization of human phosphatidylserine synthases 1 and 2

Tomohiro, S.; Kawaguti, A.; Kawabe, Y.; Kitada, S.; Kuge, O.; Biochem. J. 418, 421-429 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.8.29
expression in HeLa cell
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.29
phosphatidylserine
product inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.29
0.12
-
L-serine
pH 7.5, 37░C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.29
Ca2+
required
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
50000
-
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.29
Homo sapiens
Q9BVG9
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.29
epitope-tagged recombinant protein
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.29
1
-
pH 7.5, 37░C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717210
Homo sapiens
L-1-phosphatidylserine + ethanolamine
-
-
-
?
2.7.8.29
additional information
no substrate: phosphatidylcholine
717210
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.29
7.5
-
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
expression in HeLa cell
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.29
phosphatidylserine
product inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.29
0.12
-
L-serine
pH 7.5, 37░C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.29
Ca2+
required
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.29
50000
-
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
epitope-tagged recombinant protein
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.29
1
-
pH 7.5, 37░C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717210
Homo sapiens
L-1-phosphatidylserine + ethanolamine
-
-
-
?
2.7.8.29
additional information
no substrate: phosphatidylcholine
717210
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.29
?
x * 50000, SDS-PAGE of epitope-tagged recombinant protein
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.29
7.5
-
-
Homo sapiens