BRENDA - Enzyme Database

Cloning and expression of murine liver phosphatidylserine synthase (PSS)-2: differential regulation of phospholipid metabolism by PSS1 and PSS2

Stone, S.J.; Vance, J.E.; Biochem. J. 342, 57-64 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.8.29
expression in McArdle cell and in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, EC 2.7.8.29
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.29
Mus musculus
Q9Z1X2
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.29
liver
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717208
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.29
expression in McArdle cell and in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, EC 2.7.8.29
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.29
liver
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.29
L-1-phosphatidylethanolamine + L-serine
-
717208
Mus musculus
L-1-phosphatidylserine + ethanolamine
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
2.7.8.29
physiological function
expression of PSS2 in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, reverses the ethanolamine auxotrophy. However, the phosphatidylethanolamine content is not normalized unless the culture medium is supplemented with ethanolamine. In both mutant chinese hamster ovary cells and hepatoma cells transfected with PSS2 cDNA the rate of synthesis of phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine does not exceed that in parental chinese hamster ovary cells or control McArdle cells, respectively. Expression of murine PSS2 in McArdle cells does not inhibit phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway, whereas expression of similar levels of PSS1 activity inhibit this pathway by approx. 50%
Mus musculus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.8.29
physiological function
expression of PSS2 in ethanolamine-requiring mutant Chinese hamster ovary cells defective in PSS1, reverses the ethanolamine auxotrophy. However, the phosphatidylethanolamine content is not normalized unless the culture medium is supplemented with ethanolamine. In both mutant chinese hamster ovary cells and hepatoma cells transfected with PSS2 cDNA the rate of synthesis of phosphatidylserine and phosphatidylserine-derived phosphatidylethanolamine does not exceed that in parental chinese hamster ovary cells or control McArdle cells, respectively. Expression of murine PSS2 in McArdle cells does not inhibit phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway, whereas expression of similar levels of PSS1 activity inhibit this pathway by approx. 50%
Mus musculus