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Literature summary extracted from

  • Large, P.; Boulton, C.; Crabbe, M.
    The reduced nicotinamide-adenine dinucleotide phosphate- and oxygen-dependent N-oxygenation of trimethylamine by Pseudomonas aminovorans (1972), Biochem. J., 128, 137P-138P.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.148 3-dimethylaminopropan-1-ol inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 3-dimethylaminopropan-2-ol inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 Ca2+
-
 Aminobacter aminovorans
1.14.13.148 Co2+
-
 Aminobacter aminovorans
1.14.13.148 diethylmethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 ethyldimethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 additional information the enzyme is not inhibited by CO, cyanide or SKF 525-A (2-diethylaminoethyl 2,2-diphenylvalerate hydrochloride), nor by chelating agents Aminobacter aminovorans
1.14.13.148 N,N-dimethyl-2-chloroethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 N,N-dimethylethanolamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 N,N-dimethylethylenediamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 N,N-dimethylpropylenediamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 triethylamine inhibitory at concentrations above about 1 mM Aminobacter aminovorans
1.14.13.148 Zn2+
-
 Aminobacter aminovorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.148 0.016
-
 NADPH pH and temperature not specified in the publication Aminobacter aminovorans
1.14.13.148 2
-
 NADH pH and temperature not specified in the publication Aminobacter aminovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.148 additional information the enzyme shows no metal ion requirement Aminobacter aminovorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.148 Aminobacter aminovorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.148 3-dimethylaminopropan-1-ol + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 3-dimethylaminopropan-2-ol + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 diethylmethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 ethyldimethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADH + H+ + O2
-
 Aminobacter aminovorans N,N,N-trimethylamine N-oxide + NAD+ + H2O
-
 ?
1.14.13.148 N,N,N-trimethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans N,N,N-trimethylamine N-oxide + NADP+ + H2O
-
 ?
1.14.13.148 N,N-dimethyl-2-chloroethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 N,N-dimethylethanolamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 N,N-dimethylethylenediamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 N,N-dimethylpropylenediamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?
1.14.13.148 triethylamine + NADPH + H+ + O2
-
 Aminobacter aminovorans ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.148 35
-
 the enzyme has a half-life in the crude extract of 20 min at 35°C Aminobacter aminovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.148 4 9 the enzyme is stable at 0°C for 30min at any pH value in the range 4.0-9.0 Aminobacter aminovorans

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.148 FAD
-
 Aminobacter aminovorans
1.14.13.148 NADPH dependent on, preferred electron donor over NADH Aminobacter aminovorans