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Literature summary extracted from

  • Asakawa, S.; Sauer, K.; Liesack, W.; Thauer, R.
    Tetramethylammonium:coenzyme M methyltransferase system from Methanococcoides sp. (1998), Arch. Microbiol., 170, 220-226.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.253 Ti(III) citrate dependent on Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.252 Mg2+
-
 Methanosarcina barkeri
2.1.1.253 Mg2+
-
 Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.252 50000
-
 x * 50000, SDS-PAGE Methanosarcina barkeri
2.1.1.253 40000
-
 x * 40000, SDS-PAGE Methanosarcina barkeri

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.247 Methanosarcina barkeri
-
 gene mtbA
-
2.1.1.247 Methanosarcina barkeri NaT1
-
 gene mtbA
-
2.1.1.252 Methanosarcina barkeri
-
 gene mtqB
-
2.1.1.252 Methanosarcina barkeri NaT1
-
 gene mtqB
-
2.1.1.253 Methanosarcina barkeri
-
 gene mtqA
-
2.1.1.253 Methanosarcina barkeri NaT1
-
 gene mtqA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.252 native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography Methanosarcina barkeri
2.1.1.253 native enzyme by ultracentrifugation, a first step of anion exchange chromatography, hydroxyapatite chromatography, a second step of anion exchange chromatography, adsorption and again a third step of anion exchange chromatography Methanosarcina barkeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.247 culture condition:trimethylamine-grown cell
-
 Methanosarcina barkeri
-
2.1.1.247 additional information cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity Methanosarcina barkeri
-
2.1.1.253 culture condition:tetramethylammonium-grown cell
-
 Methanosarcina barkeri
-
2.1.1.253 additional information cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity Methanosarcina barkeri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.247 0.2
-
 trimethylamine:coenzyme M methyltransferase activity, pH 7.0, 37°C Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.247 additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin Methanosarcina barkeri ?
-
 ?
2.1.1.247 additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin Methanosarcina barkeri NaT1 ?
-
 ?
2.1.1.247 [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
-
 ?
2.1.1.247 [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
-
 Methanosarcina barkeri NaT1 methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
-
 ?
2.1.1.252 tetramethylammonium + a [Co(I) tetramethylammonium-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) tetramethylammonium-specific corrinoid protein] + trimethylamine
-
 ?
2.1.1.252 tetramethylammonium + a [Co(I) tetramethylammonium-specific corrinoid protein]
-
 Methanosarcina barkeri NaT1 a [methyl-Co(III) tetramethylammonium-specific corrinoid protein] + trimethylamine
-
 ?
2.1.1.253 additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation Methanosarcina barkeri ?
-
 ?
2.1.1.253 additional information the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Tetramethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation Methanosarcina barkeri NaT1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.252 ? x * 50000, SDS-PAGE Methanosarcina barkeri
2.1.1.253 ? x * 40000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.247 mtbA
-
 Methanosarcina barkeri
2.1.1.247 trimethylamine:coenzyme M methyltransferase
-
 Methanosarcina barkeri
2.1.1.252 MtqB
-
 Methanosarcina barkeri
2.1.1.253 MtqA
-
 Methanosarcina barkeri
2.1.1.253 tetramethylammonium:coenzyme M methyltransferase
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.247 37
-
 assay at Methanosarcina barkeri
2.1.1.253 37
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.247 7
-
 assay at Methanosarcina barkeri
2.1.1.253 7
-
 assay at Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.253 ATP dependent on Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.247 additional information cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 Methanosarcina barkeri
2.1.1.252 additional information cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 Methanosarcina barkeri
2.1.1.253 additional information cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246 Methanosarcina barkeri