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Literature summary extracted from

  • Hallahan, D.; West, J.; Wallsgrove, R.; Smiley, D.; Dawson, G.; Pickett, J.; Hamilton, J.
    Purification and characterization of an acyclic monoterpene primary alcohol:NADP+ oxidoreductase from catmint (Nepeta racemosa) (1995), Arch. Biochem. Biophys., 318, 105-112.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.324 additional information plant (4aR,7S,7aS)-nepetalactone can be used for synthesis of (4aR,7S,7aS)-nepetalactol, an aphid pheromone, useful in aphid control Nepeta racemosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.324 1,10-phenanthroline 79% inhibition at 1.0 mM, 87% at 10 mM Nepeta racemosa
1.1.1.324 4-chloromercuribenzoate complete inhibition at 1 mM and above Nepeta racemosa
1.1.1.324 EDTA 44% inhibition at 100 mM Nepeta racemosa
1.1.1.324 iodoacetamide 5% inhibition at 1.0 mM, 22% at 10 mM Nepeta racemosa
1.1.1.324 NEM 55% inhibition at 1.0 mM, complete inhibition at 10 mM Nepeta racemosa
1.1.1.324 Triton X-100 potent inhibition at 0.01-0.1% Nepeta racemosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.324 0.018
-
 NADP+ with 8-hydroxynerol, pH 9.5, 25°C Nepeta racemosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.324 soluble
-
 Nepeta racemosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.324 Zn2+ the enzyme contains zinc ions Nepeta racemosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.324 40000
-
 1 * 40000 + 1 x 42000, SDS-PAGE Nepeta racemosa
1.1.1.324 63000
-
 gel filtration Nepeta racemosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.324 (6E)-8-hydroxygeraniol + 2 NADP+ Nepeta racemosa
-
 (6E)-8-oxogeranial + 2 NADPH + 2 H+
-
 ?
1.1.1.324 (6E)-8-hydroxynerol + 2 NADP+ Nepeta racemosa
-
 ? + 2 NADPH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.324 Nepeta racemosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.324 native enzyme 1149fold from leaves to homogeneity by ultracentrifugation, ammonium sulfate fractionation, hydrophobic inetraction and anion exchange chromatography, affinity chromatography, and gel filtration Nepeta racemosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.324 leaf
-
 Nepeta racemosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.324 1417
-
 purified enzyme, pH 9.5, 25°C Nepeta racemosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.324 (6E)-8-hydroxygeraniol + 2 NADP+
-
 Nepeta racemosa (6E)-8-oxogeranial + 2 NADPH + 2 H+
-
 ?
1.1.1.324 (6E)-8-hydroxygeraniol + 2 NADP+ oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis Nepeta racemosa (6E)-8-oxogeranial + 2 NADPH + 2 H+
-
 ?
1.1.1.324 (6E)-8-hydroxynerol + 2 NADP+
-
 Nepeta racemosa ? + 2 NADPH + 2 H+
-
 ?
1.1.1.324 (6E)-8-hydroxynerol + 2 NADP+ oxidization at C1 and C8, depending on numbering of the molecule, GC-MS product analysis Nepeta racemosa ? + 2 NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.324 dimer 1 * 40000 + 1 x 42000, SDS-PAGE Nepeta racemosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.324 acyclic monoterpene primary alcohol:NADP+ oxidoreductase
-
 Nepeta racemosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.324 25
-
 assay at Nepeta racemosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.324 9.5
-
-
 Nepeta racemosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.324 additional information no activity with NAD+ Nepeta racemosa
1.1.1.324 NADP+ dependent on Nepeta racemosa

General Information

EC Number General Information Comment Organism
1.1.1.324 metabolism the enzyme plays a role in the biosynthesis of iridoid monoterpenes, e.g. (4aR,7S,7aS)-nepetalactone Nepeta racemosa