EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.160 | expression in Escherichia coli | Pseudomonas putida |
1.14.14.155 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.160 | 0.028 | - |
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one | pH 9.0, 20°C | Pseudomonas putida | |
1.14.13.160 | 0.127 | - |
Cyclobutanone | pH 9.0, 20°C | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.160 | 60000 | - |
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
1.14.13.160 | 63500 | - |
x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
1.14.14.155 | 40000 | - |
x * 40000, SDS-PAGE | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.160 | Pseudomonas putida | H3JQW0 | - |
- |
1.14.13.160 | Pseudomonas putida NCIMB 10007 | H3JQW0 | - |
- |
1.14.14.155 | Pseudomonas putida | D7UER1 | - |
- |
1.14.14.155 | Pseudomonas putida NCIMB 10007 | D7UER1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.160 | recombinant enzyme | Pseudomonas putida |
1.14.14.155 | HisTrap column chromatography | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.160 | 2200 | - |
pH 9.0, 20°C | Pseudomonas putida |
1.14.14.155 | 0.09 | - |
crude extract, at pH 7.5 and 25°C | Pseudomonas putida |
1.14.14.155 | 0.46 | - |
purified enzyme, at pH 7.5 and 25°C | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.160 | 4°C, 24 h, 50% loss of activity | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.160 | (+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | (+-)-cis-bicyclo[3.2.0]hept-2-en-6-one + O2 + NADPH + H+ | - |
Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 34% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 2,3,4,5-tetramethyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 34% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 2-cyclohexen-1-one + O2 + NADPH + H+ | 74% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 2-cyclohexen-1-one + O2 + NADPH + H+ | 74% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 2-cyclopenten-1-one + O2 + NADPH + H+ | 62% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 22% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 3,5,5-trimethyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 22% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 3-methyl-2-cyclohexen-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida NCIMB 10007 | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | 3-methyl-2-cyclopenten-1-one + O2 + NADPH + H+ | 13% conversion | Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | cyclobutanone + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.13.160 | norcamphor + O2 + NADPH + H+ | - |
Pseudomonas putida | ? + NADP+ + H2O | - |
? | |
1.14.14.155 | (+)-camphor + FMNH2 + O2 | 88% conversion | Pseudomonas putida | ? + FMN + H2O | - |
? | |
1.14.14.155 | (+)-camphor + FMNH2 + O2 | 88% conversion | Pseudomonas putida NCIMB 10007 | ? + FMN + H2O | - |
? | |
1.14.14.155 | (+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 | 99% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | (+/-)-cis-bicyclo[3.2.0]hept-2-en-6-one + FMNH2 + O2 | 99% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
1.14.14.155 | (-)-camphor + FMNH2 + O2 | 91% conversion | Pseudomonas putida | ? + FMN + H2O | - |
? | |
1.14.14.155 | (-)-camphor + FMNH2 + O2 | 91% conversion | Pseudomonas putida NCIMB 10007 | ? + FMN + H2O | - |
? | |
1.14.14.155 | (R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 | 26% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | (R,R)-bicyclo[2.2.1]heptane-2,5-dione + FMNH2 + O2 | 26% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
1.14.14.155 | 2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 | 43% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 2,3,4,5-tetramethyl-2-cyclopenten-1-one + FMNH2 + O2 | 43% conversion | Pseudomonas putida NCIMB 10007 | ? | - |
? | |
1.14.14.155 | 2-cyclohexen-1-one + FMNH2 + O2 | 50% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 2-cyclopenten-1-one + FMNH2 + O2 | 48% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 2-decanone + FMNH2 + O2 | 11% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 3,5,5-trimethyl-2-cyclohexen-1-one + FMNH2 + O2 | 21% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 3-methyl-2-cyclohexen-1-one + FMNH2 + O2 | 20% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 3-methyl-2-cyclopenten-1-one + FMNH2 + O2 | 10% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | 4-phenyl-2-butanone + FMNH2 + O2 | 48% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | acetophenone + FMNH2 + O2 | 80% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | cyclobutanone + FMNH2 + O2 | 13% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | cyclohexanone + FMNH2 + O2 | 3% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | cyclopentanone + FMNH2 + O2 | 24% conversion | Pseudomonas putida | ? | - |
? | |
1.14.14.155 | norcamphor + FMNH2 + O2 | 77% conversion | Pseudomonas putida | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.160 | ? | x * 60000, SDS-PAGE, x * 63500, calculated, both for His-tagged protein | Pseudomonas putida |
1.14.14.155 | ? | x * 40000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.155 | 3,6-diketocamphane 1,6-monooxygenase | - |
Pseudomonas putida |
1.14.14.155 | 3,6-DKCMO | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.160 | 23 | - |
- |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.160 | 20 | - |
half-life 196 min | Pseudomonas putida |
1.14.13.160 | 25 | - |
half-life 43 min | Pseudomonas putida |
1.14.13.160 | 30 | - |
half-life 9 min | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.160 | 9 | - |
- |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.160 | FAD | - |
Pseudomonas putida | |
1.14.14.155 | FMN | dependent on | Pseudomonas putida | |
1.14.14.155 | additional information | dependent on | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.160 | 14.7 | - |
Cyclobutanone | pH 9.0, 20°C | Pseudomonas putida | |
1.14.13.160 | 49.3 | - |
(+-)-cis-bicyclo[3.2.0]hept-2-en-6-one | pH 9.0, 20°C | Pseudomonas putida |