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Literature summary extracted from

  • Bao, L.; Huang, Q.; Chang, L.; Sun, Q.; Zhou, J.; Lu, H.
    Cloning and characterization of two beta-glucosidase/xylosidase enzymes from yak rumen metagenome (2012), Appl. Biochem. Biotechnol., 166, 72-86.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 ethanol 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 methanol 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 ethanol 5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 methanol 5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expressed in Escherichia coli and purified uncultured rumen bacterium
3.2.1.74 expressed in Escherichia coli and purified uncultured rumen bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.37 molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium
3.2.1.37 molecular docking studies for the enzyme with the substrates xylobiose and 4-nitrophenyl-beta-D-xylopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium
3.2.1.74 molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl beta-D-glucopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are conservative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium
3.2.1.74 molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl-beta-D-glucopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters uncultured rumen bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.1317
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 65
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 0.22
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 1.06
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 40°C uncultured rumen bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 CaCl2 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 CaCl2 5 mM, 99% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 CoCl2 5 mM, 89.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 CoCl2 5 mM, 91.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 CuCl2 5 mM, 87.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 CuCl2 5 mM, 96.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 D-fructose 5 mM, 76.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 D-fructose 5 mM, 86.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 D-glucose 5 mM, 4.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 D-glucose 5 mM, 54.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 D-xylose 5 mM, 81.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 D-xylose 5 mM, 97.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 MgCl2 5 mM, 87.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 MgCl2 5 mM, 94.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 MnCl2 5 mM, 93.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 MnCl2 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 NiCl2 5 mM, 96.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 NiCl2 5 mM, 98.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 ZnSO4 5 mM, 101.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 ZnSO4 5 mM, 99.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 CaCl2 5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 CaCl2 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 CoCl2 5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 CoCl2 5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 CuCl2 5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 CuCl2 5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 D-fructose 5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 D-glucose 5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 D-xylose 5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 fructose 5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 glucose 5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 MgCl2 5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 MgCl2 5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 MnCl2 5 mM, 102.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 MnCl2 5 mM, 112% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 NiCl2 5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 NiCl2 5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 xylose 5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 ZnSO4 5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 ZnSO4 5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.37 Ethanol 5% (v/v), 112.7% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 Ethanol 5% (v/v), 316.9% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 Methanol 5% (v/v), 167% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 Methanol 5% (v/v), 94.3% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 Ethanol 5% (v/v), 100.8% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 Ethanol 5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 Methanol 5% (v/v), 108.2% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 Methanol 5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 uncultured rumen bacterium G3BJX3 Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-
3.2.1.37 uncultured rumen bacterium G3BJX4 Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-
3.2.1.74 uncultured rumen bacterium G3BJX3 Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-
3.2.1.74 uncultured rumen bacterium G3BJX4 Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 recombinant enzyme uncultured rumen bacterium
3.2.1.74 recombinant enzyme uncultured rumen bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured rumen bacterium
-
3.2.1.74 additional information isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens uncultured rumen bacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 5.5
-
 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 12.1
-
 4-nitrophenyl beta-D-xylopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 0.35
-
 2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 0.65
-
 2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 5.3
-
 cellobiose as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 11.5
-
 4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 26
-
 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 33.5
-
 cellobiose as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 41
-
 4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 77
-
 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42°C uncultured rumen bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 15.7% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O 21.1% relative activity compared to the beta-glucosidase activity with 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 additional information RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone uncultured rumen bacterium ?
-
 ?
3.2.1.37 additional information RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan uncultured rumen bacterium ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 uncultured rumen bacterium 2 D-xylose
-
 ?
3.2.1.74 2-nitrophenyl beta-D-galactopyranoside + H2O 0.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 2-nitrophenol + D-galactose
-
 ?
3.2.1.74 2-nitrophenyl beta-D-galactopyranoside + H2O 1.3% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 2-nitrophenol + D-galactose
-
 ?
3.2.1.74 4-methylumbelliferyl beta-D-cellobioside + 2 H2O i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate uncultured rumen bacterium 4-methylumbelliferol + 2 D-glucose
-
 ?
3.2.1.74 4-methylumbelliferyl-beta-D-cellobioside + H2O i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium D-glucose + ?
-
 ?
3.2.1.74 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 uncultured rumen bacterium 4-nitrophenol + D-glucose
-
 ?
3.2.1.74 cellobiose + H2O 128.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium 2 D-glucose cellobiose is completely hydrolyzed to glucose, evaluated by chromatographic analysis ?
3.2.1.74 cellobiose + H2O 6.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate uncultured rumen bacterium 2 D-glucose 31.5% cellobiose is hydrolyzed to glucose, evaluated by chromatographic analysis ?
3.2.1.74 cellotetraose + H2O
-
 uncultured rumen bacterium glucose + cellobiose + cellotriose with a conversion ratio of 80% ?
3.2.1.74 additional information no activity with 4-nitrophenyl alpha-D-glucopyranoside and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates uncultured rumen bacterium ?
-
 ?
3.2.1.74 additional information no activity with 4-nitrophenyl alpha-D-glucopyranoside, cellotetraose, and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates uncultured rumen bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-glucosidase/xylosidase bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3 uncultured rumen bacterium
3.2.1.37 beta-xylosidase
-
 uncultured rumen bacterium
3.2.1.37 Rubg3A gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium
3.2.1.37 Rubg3B gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium
3.2.1.74 beta-glucosidase
-
 uncultured rumen bacterium
3.2.1.74 beta-glucosidase/xylosidase bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3 uncultured rumen bacterium
3.2.1.74 Rubg3A gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium
3.2.1.74 Rubg3B gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74 uncultured rumen bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 35
-
 optimal, with 4-nitrophenyl-beta-D-xylopyranoside as substrate uncultured rumen bacterium
3.2.1.37 40
-
 assay at uncultured rumen bacterium
3.2.1.37 40
-
 optimal, with 4-nitrophenyl-beta-D-xylopyranoside uncultured rumen bacterium
3.2.1.37 42
-
 assay at uncultured rumen bacterium
3.2.1.74 40
-
 assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside uncultured rumen bacterium
3.2.1.74 42
-
 assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 43
-
 Tm-value uncultured rumen bacterium
3.2.1.74 45
-
 40% activity retained after incubation for 1 h uncultured rumen bacterium
3.2.1.74 45
-
 stable after 1 h incubation uncultured rumen bacterium
3.2.1.74 50
-
 Tm-value, retained 50% activity after 20 min incubation uncultured rumen bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 17
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium
3.2.1.37 135
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 126.6
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 203
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 42°C uncultured rumen bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4.6
-
 assay at uncultured rumen bacterium
3.2.1.37 5.5
-
 optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate uncultured rumen bacterium
3.2.1.37 7.5
-
 optimal, with 4-nitrophenyl beta-D-xylopyranoside as substrate uncultured rumen bacterium
3.2.1.74 4.6
-
 assay at, optimal for 4-nitrophenol beta-D-glucopyranoside as substrate uncultured rumen bacterium
3.2.1.74 4.6
-
 assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate uncultured rumen bacterium

General Information

EC Number General Information Comment Organism
3.2.1.37 physiological function RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3A with an activity ratio of 10:1 (xylosidase/xylanase) increases the liberation to 218% of that in the presence of XynR8 alone uncultured rumen bacterium
3.2.1.37 physiological function RuBG3A or RuBG3B alone do not release any reducing sugars from xylan. The addition of each enzyme into the reaction mixture of xylanase XynR8 improves the liberation of reducing sugars. The addition of RuBG3B with an activity ratio of 5:1 (xylosidase/xylanase) increases the liberation to 169% of that in the presence of XynR8 alone. RuBG3B shows no synergism with xylanase XynR8 at the optimal pH 7.5, but at pH 5.5, indicating that RuBG3B may have different pH preferences between aryl xylosidic substrate 4-nitrophenyl-beta-D-xylopyranoside and xylooligisaccharide xylan uncultured rumen bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 2084
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 40°C uncultured rumen bacterium
3.2.1.37 54080
-
 4-nitrophenyl beta-D-xylopyranoside pH 4.6, 42°C uncultured rumen bacterium
3.2.1.74 11900
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 40°C uncultured rumen bacterium
3.2.1.74 915700
-
 4-nitrophenyl beta-D-glucopyranoside pH 4.6, 42°C uncultured rumen bacterium