Literature summary extracted from
Nogly, P.; Castro, R.; de Rosa, M.; Neves, A.R.; Santos, H.; Archer, M.
Production and crystallization of alpha-phosphoglucomutase from Lactococcus lactis (2012), Acta Crystallogr. Sect. F, 68, 1113-1115.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.2.2 |
cloned and homologously overexpressed in Lactococcus lactis subsp. cremoris strain NZ9000, introducing 6xHis-tag |
Lactococcus lactis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.4.2.2 |
crystallized with vapor diffusion, streak seeding, in ammonium sulfate solution, optimized crystals diffract to 1.5 A resolution |
Lactococcus lactis |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.4.2.2 |
29880 |
- |
calculated |
Lactococcus lactis |
5.4.2.2 |
30000 |
- |
SDS-PAGE of purified alpha-PGM, with His-tag |
Lactococcus lactis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.2.2 |
Lactococcus lactis |
Q00G41 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.2.2 |
recombinant protein is purified by centrifugation und affinity chromatography with Ni-NTA Superflow cartridge. Purity is assessed by SDS-PAGE |
Lactococcus lactis |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
5.4.2.2 |
-80°C, fast-frozen in LN2 and stored for 2 years without affecting the sample homogeneity |
Lactococcus lactis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.2.2 |
alpha-Pgm |
- |
Lactococcus lactis |
5.4.2.2 |
alpha-phosphoglucomutase |
- |
Lactococcus lactis |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
5.4.2.2 |
physiological function |
Unlike the majority of alpha-phosphoglucomutases, the described enzyme of 252 residues with alpha-phosphoglucomutase activity, is related to eukaryotic phosphomannomutases and belongs to HAD-superfamily hydrolase, subfamily IIB. |
Lactococcus lactis |