Literature summary extracted from
Zhang, Y.; Edwards, T.E.; Begley, D.W.; Abramov, A.; Thompkins, K.B.; Ferrell, M.; Guo, W.J.; Phan, I.; Olsen, C.; Napuli, A.; Sankaran, B.; Stacy, R.; Van Voorhis, W.C.; Stewart, L.J.; Myler, P.J.
Structure of nitrilotriacetate monooxygenase component B from Mycobacterium thermoresistibile (2011), Acta Crystallogr. Sect. F, 67, 1100-1105.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.14.14.10 |
expressed in Escherichia coli BL21(DE3) Rosetta cells |
Mycolicibacterium thermoresistibile |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.14.14.10 |
sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000 |
Mycolicibacterium thermoresistibile |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.14.10 |
Mycolicibacterium thermoresistibile |
E5Q9D7 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.14.10 |
HisTrap FF column chromatography and Superdex 75 gel filtration |
Mycolicibacterium thermoresistibile |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.14.14.10 |
nitrilotriacetate + FMNH2 + H+ + O2 |
- |
Mycolicibacterium thermoresistibile |
iminodiacetate + glyoxylate + FMN + H2O |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.14.14.10 |
homodimer |
x-ray crystallography |
Mycolicibacterium thermoresistibile |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.14.10 |
nitrilotriacetate monooxygenase component B |
- |
Mycolicibacterium thermoresistibile |
1.14.14.10 |
NTA-MoB |
- |
Mycolicibacterium thermoresistibile |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.14.14.10 |
FMNH2 |
- |
Mycolicibacterium thermoresistibile |
|