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Literature summary extracted from

  • Kim, K.J.; Kim, S.; Lee, S.; Kang, B.S.; Lee, H.S.; Oh, T.K.; Kim, M.H.
    Crystallization and initial crystallographic characterization of the Corynebacterium glutamicum nitrilotriacetate monooxygenase component A (2006), Acta Crystallogr. Sect. F, 62, 1141-1143.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.10 sitting drop vapor diffusion method, using 1.6 M NH2SO4, 0.1 M NaCl and 0.1 M HEPES pH 7.5 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.10 Mg2+ required for activity Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.10 47800
-
 2 * 47800, estimated from SDS-PAGE Corynebacterium glutamicum
1.14.14.10 100000
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.10 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.10 Ni-NTA column chromatography, HiTrap Q anion-exchange column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.10 nitrilotriacetate + FMNH2 + H+ + O2
-
 Corynebacterium glutamicum iminodiacetate + glyoxylate + FMN + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.14.14.10 homodimer 2 * 47800, estimated from SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
1.14.14.10 nitrilotriacetate monooxygenase component A
-
 Corynebacterium glutamicum
1.14.14.10 NTA MO A
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.10 FMNH2
-
 Corynebacterium glutamicum