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Literature summary extracted from
- Cloning, expression and characterization of alkaline phosphatase from a thermophilic bacterium Geobacillus caldoxylosilyticus TK4 (2010), Turk. J. Biochem., 35, 208-214.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.1 | expressed in Escherichia coli as a His-tagged fusion protein | Parageobacillus caldoxylosilyticus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | EDTA | - |
Parageobacillus caldoxylosilyticus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.1 | 0.087 | - |
4-nitrophenyl phosphate | pH 9.5, 50°C, Vmax: 0.049 micromol/min/mg | Parageobacillus caldoxylosilyticus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | Al3+ | inhibitory effect | Parageobacillus caldoxylosilyticus | |
| 3.1.3.1 | Cd2+ | inhibitory effect | Parageobacillus caldoxylosilyticus | |
| 3.1.3.1 | Hg2+ | inhibitory effect | Parageobacillus caldoxylosilyticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 50000 | - |
SDS-PAGE | Parageobacillus caldoxylosilyticus |
| 3.1.3.1 | 51590 | - |
calculated from cDNA | Parageobacillus caldoxylosilyticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Parageobacillus caldoxylosilyticus | C1K6P2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.1 | using Ni-NTA chromatography | Parageobacillus caldoxylosilyticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | 4-nitrophenyl phosphate + H2O | - |
Parageobacillus caldoxylosilyticus | 4-nitrophenol + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | alkaline phosphatase | - |
Parageobacillus caldoxylosilyticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 50 | - |
- |
Parageobacillus caldoxylosilyticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 10 | 30 | 100% active for 2 h | Parageobacillus caldoxylosilyticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 9.5 | - |
- |
Parageobacillus caldoxylosilyticus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 9.5 | - |
100% active after 12 h incubation at 4°C | Parageobacillus caldoxylosilyticus |
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