Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Valiyaveettil, M.; Alamneh, Y.; Biggemann, L.; Soojhawon, I.; Farag, H.A.; Agrawal, P.; Doctor, B.P.; Nambiar, M.P.
    In vitro efficacy of paraoxonase 1 from multiple sources against various organophosphates (2011), Toxicol. In Vitro, 25, 905-913.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 expressed in Trichoplusia ni larvae Homo sapiens
3.1.8.1 expressed in Trichoplusia ni larvae Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 2-hydroxyquinoline dose-dependent inhibition of enzyme activity against 4-nitrophenyl acetate substrate Homo sapiens
3.1.1.2 2-hydroxyquinoline dose-dependent inhibition of enzyme activity against 4-nitrophenyl acetate substrate Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.289
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.1.2 0.336
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus
3.1.1.2 0.611
-
4-nitrophenyl acetate recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 0.67
-
diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 0.772
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 0.889
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 Ca2+ required Homo sapiens
3.1.1.2 Ca2+ required Oryctolagus cuniculus
3.1.8.1 Ca2+ required Homo sapiens
3.1.8.1 Ca2+ required Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens
-
-
-
3.1.1.2 Oryctolagus cuniculus
-
-
-
3.1.8.1 Homo sapiens
-
-
-
3.1.8.1 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2 Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Homo sapiens
3.1.1.2 Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Oryctolagus cuniculus
3.1.8.1 Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Homo sapiens
3.1.8.1 Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 serum
-
Homo sapiens
-
3.1.1.2 serum
-
Oryctolagus cuniculus
-
3.1.8.1 serum
-
Homo sapiens
-
3.1.8.1 serum
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 4-nitrophenyl acetate + H2O
-
Homo sapiens 4-nitrophenol + acetate
-
?
3.1.1.2 4-nitrophenyl acetate + H2O
-
Oryctolagus cuniculus 4-nitrophenol + acetate
-
?
3.1.8.1 diethyl paraoxon + H2O
-
Homo sapiens ?
-
?
3.1.8.1 diethyl paraoxon + H2O
-
Oryctolagus cuniculus ?
-
?
3.1.8.1 diisopropyl fluorophosphate + H2O high concentration of purified human serum paraoxonase 1 (about 0.075 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates Homo sapiens ?
-
?
3.1.8.1 diisopropyl fluorophosphate + H2O high concentration of purified rabbit serum paraoxonase 1 (about 0.025 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates Oryctolagus cuniculus ?
-
?
3.1.8.1 dimethyl paraoxon + H2O
-
Homo sapiens ?
-
?
3.1.8.1 dimethyl paraoxon + H2O
-
Oryctolagus cuniculus ?
-
?
3.1.8.1 additional information recombinant human paraoxoanase 1 cannot hydrolyze dimethyl paraoxon even at higher concentrations used Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 ? x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in the about 40000-45000 kDa molecular weight regions, SDS-PAGE Oryctolagus cuniculus
3.1.1.2 ? x * 42000-45000, purified recombinant human paraoxonase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE Homo sapiens
3.1.8.1 ? x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in about 40000-45000 Da molecular weight regions, SDS-PAGE Oryctolagus cuniculus
3.1.8.1 ? x * 42000-45000, purified recombinant human paraoxoanase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.2 1300
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.1.2 2500
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus
3.1.1.2 2500
-
4-nitrophenyl acetate recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 4.3
-
diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 5.7
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 16.2
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.2 4200
-
4-nitrophenyl acetate recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.1.2 4700
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.1.2 7500
-
4-nitrophenyl acetate in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus
3.1.8.1 1.8
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus
3.1.8.1 6.5
-
diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
3.1.8.1 7.3
-
diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens