EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.2 | expressed in Trichoplusia ni larvae | Homo sapiens |
3.1.8.1 | expressed in Trichoplusia ni larvae | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.2 | 2-hydroxyquinoline | dose-dependent inhibition of enzyme activity against 4-nitrophenyl acetate substrate | Homo sapiens | |
3.1.1.2 | 2-hydroxyquinoline | dose-dependent inhibition of enzyme activity against 4-nitrophenyl acetate substrate | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 0.289 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.1.2 | 0.336 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus | |
3.1.1.2 | 0.611 | - |
4-nitrophenyl acetate | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 0.67 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 0.772 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 0.889 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.2 | Ca2+ | required | Homo sapiens | |
3.1.1.2 | Ca2+ | required | Oryctolagus cuniculus | |
3.1.8.1 | Ca2+ | required | Homo sapiens | |
3.1.8.1 | Ca2+ | required | Oryctolagus cuniculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.2 | Homo sapiens | - |
- |
- |
3.1.1.2 | Oryctolagus cuniculus | - |
- |
- |
3.1.8.1 | Homo sapiens | - |
- |
- |
3.1.8.1 | Oryctolagus cuniculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.2 | Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Homo sapiens |
3.1.1.2 | Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Oryctolagus cuniculus |
3.1.8.1 | Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Homo sapiens |
3.1.8.1 | Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.2 | serum | - |
Homo sapiens | - |
3.1.1.2 | serum | - |
Oryctolagus cuniculus | - |
3.1.8.1 | serum | - |
Homo sapiens | - |
3.1.8.1 | serum | - |
Oryctolagus cuniculus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.2 | 4-nitrophenyl acetate + H2O | - |
Homo sapiens | 4-nitrophenol + acetate | - |
? | |
3.1.1.2 | 4-nitrophenyl acetate + H2O | - |
Oryctolagus cuniculus | 4-nitrophenol + acetate | - |
? | |
3.1.8.1 | diethyl paraoxon + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | diethyl paraoxon + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
3.1.8.1 | diisopropyl fluorophosphate + H2O | high concentration of purified human serum paraoxonase 1 (about 0.075 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates | Homo sapiens | ? | - |
? | |
3.1.8.1 | diisopropyl fluorophosphate + H2O | high concentration of purified rabbit serum paraoxonase 1 (about 0.025 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates | Oryctolagus cuniculus | ? | - |
? | |
3.1.8.1 | dimethyl paraoxon + H2O | - |
Homo sapiens | ? | - |
? | |
3.1.8.1 | dimethyl paraoxon + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
3.1.8.1 | additional information | recombinant human paraoxoanase 1 cannot hydrolyze dimethyl paraoxon even at higher concentrations used | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.2 | ? | x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in the about 40000-45000 kDa molecular weight regions, SDS-PAGE | Oryctolagus cuniculus |
3.1.1.2 | ? | x * 42000-45000, purified recombinant human paraoxonase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE | Homo sapiens |
3.1.8.1 | ? | x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in about 40000-45000 Da molecular weight regions, SDS-PAGE | Oryctolagus cuniculus |
3.1.8.1 | ? | x * 42000-45000, purified recombinant human paraoxoanase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 1300 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.1.2 | 2500 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus | |
3.1.1.2 | 2500 | - |
4-nitrophenyl acetate | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 4.3 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 5.7 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 16.2 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.2 | 4200 | - |
4-nitrophenyl acetate | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.1.2 | 4700 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.1.2 | 7500 | - |
4-nitrophenyl acetate | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus | |
3.1.8.1 | 1.8 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus | |
3.1.8.1 | 6.5 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
3.1.8.1 | 7.3 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens |