BRENDA - Enzyme Database

Structure of an archaeal-type phosphoenolpyruvate carboxylase sensitive to inhibition by aspartate

Dharmarajan, L.; Kraszewski, J.L.; Mukhopadhyay, B.; Dunten, P.W.; Proteins 79, 1820-1829 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
additional information
not activated by D-glucose 6-phosphate
Clostridium perfringens
Cloned(Commentary)
EC Number
Commentary
Organism
4.1.1.31
expressed in Escherichia coli
Clostridium perfringens
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.1.1.31
hanging drop vapor diffusion method, using 1.25-1.5 M sodium malonate, pH 7.0, as precipitant
Clostridium perfringens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
L-Asp
L-Asp competitively inhibits the enzyme with respect to the substrate, Mg2+-phosphoenolpyruvate
Clostridium perfringens
4.1.1.31
malonate
weak inhibitor
Clostridium perfringens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.06
-
phosphoenolpyruvate
in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C
Clostridium perfringens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Co2+
PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
4.1.1.31
Mg2+
required for activity, PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
4.1.1.31
Mn2+
PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.31
Clostridium perfringens
Q8XLE8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.31
affinity chromatography and gel filtration
Clostridium perfringens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + HCO3-
-
716881
Clostridium perfringens
phosphate + oxaloacetate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.31
tetramer
X-ray crystallography, analytical ultracentrifugation, or sedimentation velocity analysis
Clostridium perfringens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.31
0.2
-
L-Asp
in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C
Clostridium perfringens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.31
additional information
not activated by D-glucose 6-phosphate
Clostridium perfringens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
expressed in Escherichia coli
Clostridium perfringens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.1.1.31
hanging drop vapor diffusion method, using 1.25-1.5 M sodium malonate, pH 7.0, as precipitant
Clostridium perfringens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
L-Asp
L-Asp competitively inhibits the enzyme with respect to the substrate, Mg2+-phosphoenolpyruvate
Clostridium perfringens
4.1.1.31
malonate
weak inhibitor
Clostridium perfringens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.1.31
0.2
-
L-Asp
in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C
Clostridium perfringens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.31
0.06
-
phosphoenolpyruvate
in the presence of 2 mM Mg2+, in 50 mM HEPES-NaOH buffer (pH 7.2), at 37°C
Clostridium perfringens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.31
Co2+
PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
4.1.1.31
Mg2+
required for activity, PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
4.1.1.31
Mn2+
PepcA catalyzes formation of oxaloacetate in the presence of Mg2+, Mn2+, or Co2+ but not in the absence of a divalent metal ion
Clostridium perfringens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.31
affinity chromatography and gel filtration
Clostridium perfringens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + HCO3-
-
716881
Clostridium perfringens
phosphate + oxaloacetate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.31
tetramer
X-ray crystallography, analytical ultracentrifugation, or sedimentation velocity analysis
Clostridium perfringens