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Literature summary extracted from
- Potential anti-bacterial drug target: structural characterization of 3,4-dihydroxy-2-butanone-4-phosphate synthase from Salmonella typhimurium LT2 (2010), Proteins, 78, 3292-3303.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | expressed in Escherichia coli as a His-tagged fusion protein | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | the crystal structures of Salmonella DHBPS in complex with sulfate, D-ribulose 5-phosphate and sulfate-zinc ion is determined at a resolution of 2.80, 2.52, and 1.86 A, respectively. Analysis of these crystal structures reveals that the acidic loop (residues 34-39) responsible for the acid-base catalysis is disordered in the absence of substrate or metal ion at the active site. Upon binding either substrate or sulfate and metal ions, the acidic loop becomes stabilized, adopts a closed conformation and interacts with the substrate | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.99.12 | 0.116 | - |
D-ribulose 5-phosphate | 37°C, pH 7.5, Vmax: 199 nmol/min/mg | Salmonella enterica subsp. enterica serovar Typhimurium |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.99.12 | Hg2+ | moderate activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.1.99.12 | Mg2+ | highest activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.1.99.12 | Ni2+ | moderate activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 23310 | - |
calculated from cDNA | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.1.99.12 | 23640 | - |
MALDI | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.1.99.12 | 50000 | - |
gel filtration, dimer in solution | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.99.12 | Salmonella enterica subsp. enterica serovar Typhimurium | P66032 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.99.12 | using Ni-NTA chromatography | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.99.12 | D-ribulose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | formate + L-3,4-dihydroxy-2-butanone-4-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | homodimer | gel filtration, 2 * 23310 | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.99.12 | 3,4-dihydroxy-2-butanone-4-phosphate synthase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.1.99.12 | DHBPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.1.99.12 | ribB | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.99.12 | 7.5 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
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