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Literature summary extracted from

  • Ronning, D.R.; Iacopelli, N.M.; Mishra, V.
    Enzyme-ligand interactions that drive active site rearrangements in the Helicobacter pylori 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase (2010), Protein Sci., 19, 2498-2510.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.9 enzyme alone, in complex with formycin A and in complex with adenine, hanging drop vapor diffusion method, using 0.1 M Tris pH 8.5 and 16% (w/v) polyethyleneglycol 8000, at 18°C Helicobacter pylori
3.2.2.9 enzyme only or in complex with formycin A or adenine, hanging drop vapor diffusion method, using 0.1 M Tris pH 8.5 and 16% (w/v) polyethyleneglycol 8000, at 18°C Helicobacter pylori
3.2.2.30 enzyme only or in complex with formycin A or adenine, hanging drop vapor diffusion method, using 0.1 M Tris pH 8.5 and 16% (w/v) polyethyleneglycol 8000, at 18°C Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.9 Tris weak inhibitor; weak inhibitor, about 20% residual activity at 250 mM Helicobacter pylori
3.2.2.30 Tris weak inhibitor, about 20% residual activity at 250 mM Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.9 0.0449
-
 5'-methylthioadenosine at 37°C in 100 mM HEPES, 50 mM KCl, pH 7.5 Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.9 S-adenosyl-L-homocysteine + H2O Helicobacter pylori
-
 S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.30 6-amino-6-deoxyfutalosine + H2O Helicobacter pylori
-
 dehypoxanthine futalosine + adenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.9 Helicobacter pylori
-
-
-
3.2.2.9 Helicobacter pylori Q9ZMY2
-
-
3.2.2.30 Helicobacter pylori Q9ZMY2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.9 HisTrap column chromatography and Superdex 200 gel filtration Helicobacter pylori
3.2.2.30 HisTrap column chromatography and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.9 5'-deoxyadenosine + H2O
-
 Helicobacter pylori 5-deoxy-D-ribose + adenine
-
 ?
3.2.2.9 5'-methylthioadenosine + H2O
-
 Helicobacter pylori 5-methylthio-D-ribose + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Helicobacter pylori S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ?
3.2.2.9 S-adenosyl-L-homocysteine + H2O
-
 Helicobacter pylori S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
-
 ir
3.2.2.30 6-amino-6-deoxyfutalosine + H2O
-
 Helicobacter pylori dehypoxanthine futalosine + adenine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.9 homodimer x-ray crystallography Helicobacter pylori
3.2.2.30 homodimer x-ray crystallography Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.2.2.9 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Helicobacter pylori
3.2.2.9 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Helicobacter pylori
3.2.2.9 MTAN
-
 Helicobacter pylori
3.2.2.30 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
 Helicobacter pylori
3.2.2.30 MTAN
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.9 4.92
-
 5'-methylthioadenosine at 37°C in 100 mM HEPES, 50 mM KCl, pH 7.5 Helicobacter pylori

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.2.9 25.24
-
 Tris at 37°C, pH 7.5 Helicobacter pylori