EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.1.14 | wild type and mutant enzymes S329F, S329K, S329L, and S329A in complex with L-rhamnose, D-psicose or D-allose, droplet vapor diffusion method, using 6-9.25% (w/v) polyethylene glycol 20000, 50 mM MES buffer, pH 6.1-6.3, at 20°C | Pseudomonas stutzeri |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.14 | S329A | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
5.3.1.14 | S329F | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
5.3.1.14 | S329K | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
5.3.1.14 | S329L | mutant showing reduced catalytic efficiency compared to the wild type enzyme | Pseudomonas stutzeri |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 7.9 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 12.2 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 18.5 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 19.4 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 24.2 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 25.9 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 33.9 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 35.6 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 39.1 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 44.4 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 44.7 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 53.3 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 65.6 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 67.3 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 85.1 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.14 | Pseudomonas stutzeri | Q75WH8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.14 | - |
Pseudomonas stutzeri |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.14 | D-allose | - |
Pseudomonas stutzeri | D-psicose | - |
r | |
5.3.1.14 | D-ribose | - |
Pseudomonas stutzeri | D-ribulose | - |
r | |
5.3.1.14 | L-Rhamnose | - |
Pseudomonas stutzeri | L-Rhamnulose | - |
r | |
5.3.1.14 | additional information | the enzyme recognizes substrates at C2 and C3 but not at C4 and C5 | Pseudomonas stutzeri | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.14 | L-RhI | - |
Pseudomonas stutzeri |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 1.7 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 1.8 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 2.7 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 3.3 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 3.8 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 5.1 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 7.4 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 8.9 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 9.8 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 11.4 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 13.3 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 21.2 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 53.6 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 142 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 224 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 0.0027 | - |
D-ribose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.0043 | - |
D-ribose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.005 | - |
D-ribose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.071 | - |
D-allose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.081 | - |
D-allose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.13 | - |
D-ribose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.2 | - |
D-ribose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.27 | - |
D-allose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.28 | - |
D-allose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.41 | - |
L-rhamnose | mutant enzyme S329K, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.47 | - |
D-allose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 0.55 | - |
L-rhamnose | mutant enzyme S329F, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 6.8 | - |
L-rhamnose | mutant enzyme S329L, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 7.7 | - |
L-rhamnose | mutant enzyme S329A, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri | |
5.3.1.14 | 11.6 | - |
L-rhamnose | wild type enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), 1 mM MnCl2, at 35°C | Pseudomonas stutzeri |