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Literature summary extracted from

  • Romao, C.V.; Ladakis, D.; Lobo, S.A.; Carrondo, M.A.; Brindley, A.A.; Deery, E.; Matias, P.M.; Pickersgill, R.W.; Saraiva, L.M.; Warren, M.J.
    Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization (2011), Proc. Natl. Acad. Sci. USA, 108, 97-102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.3
-
 Desulfovibrio vulgaris
6.6.1.2
-
 Salmonella enterica
6.6.1.2
-
 Desulfovibrio vulgaris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.99.1.3 hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate Desulfovibrio vulgaris
6.6.1.2 hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate Salmonella enterica
6.6.1.2 hanging drop vapor diffusion method, using 100 mM Tris-HCl. pH 8.5, containing 2.0 M ammonium sulfate Desulfovibrio vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.99.1.3 periplasm
-
 Archaeoglobus fulgidus
-
-
4.99.1.3 periplasm
-
 Desulfovibrio vulgaris
-
-
6.6.1.2 periplasm
-
 Salmonella enterica
-
-
6.6.1.2 periplasm
-
 Desulfovibrio vulgaris
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.99.1.4 sirohydrochlorin + Fe2+ Priestia megaterium
-
 siroheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.3 Archaeoglobus fulgidus
-
-
-
4.99.1.3 Desulfovibrio vulgaris Q72EC8
-
-
4.99.1.3 Salmonella enterica
-
-
-
4.99.1.4 Priestia megaterium
-
-
-
6.6.1.2 Desulfovibrio vulgaris
-
-
-
6.6.1.2 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.99.1.3
-
 Desulfovibrio vulgaris
6.6.1.2
-
 Salmonella enterica
6.6.1.2
-
 Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.3 sirohydrochlorin + Co2+
-
 Salmonella enterica cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+
-
 Archaeoglobus fulgidus cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.3 sirohydrochlorin + Co2+
-
 Desulfovibrio vulgaris cobalt-sirohydrochlorin + H+
-
 ?
4.99.1.4 sirohydrochlorin + Fe2+
-
 Priestia megaterium siroheme + H+
-
 ?
6.6.1.2 additional information the protein binds cobalt via residues His154, Glu184, and His216 Desulfovibrio vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.99.1.3 tetramer
-
 Salmonella enterica
4.99.1.3 tetramer
-
 Archaeoglobus fulgidus
4.99.1.3 tetramer x-ray crystallography Desulfovibrio vulgaris
6.6.1.2 tetramer
-
 Salmonella enterica
6.6.1.2 tetramer
-
 Desulfovibrio vulgaris

Synonyms

EC Number Synonyms Comment Organism
4.99.1.3 CbiK
-
 Salmonella enterica
4.99.1.3 CbiK
-
 Desulfovibrio vulgaris
4.99.1.3 CbiX
-
 Archaeoglobus fulgidus
4.99.1.4 SirB
-
 Priestia megaterium
6.6.1.2 CbiK
-
 Salmonella enterica
6.6.1.2 CbiK
-
 Desulfovibrio vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
4.99.1.3 heme heme b Salmonella enterica
4.99.1.3 heme heme b Archaeoglobus fulgidus
4.99.1.3 heme heme b Desulfovibrio vulgaris
6.6.1.2 heme b
-
 Salmonella enterica
6.6.1.2 heme b
-
 Desulfovibrio vulgaris