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Literature summary extracted from

  • Raschke, M.; Bürkle, L.; Müller, N.; Nunes-Nesi, A.; Fernie, A.R.; Arigoni, D.; Amrhein, N.; Fitzpatrick, T.B.
    Vitamin B1 biosynthesis in plants requires the essential iron sulfur cluster protein, THIC (2007), Proc. Natl. Acad. Sci. USA, 104, 19637-19642.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.99.17 chloroplast
-
 Arabidopsis thaliana 9507
-
4.1.99.17 plastid
-
 Arabidopsis thaliana 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.17 iron-sulfur centre
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.17 71994
-
 x * 71994, calculated from sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Arabidopsis thaliana the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Arabidopsis thaliana ecotype Col-0 the enzyme is involved in the biosynthesis of thiamine diphosphate 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.17 Arabidopsis thaliana O82392
-
-
4.1.99.17 Arabidopsis thaliana ecotype Col-0 O82392
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.99.17 seedling THIC transcript is not detectable two days after germination, but is readily detectable at day five Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Arabidopsis thaliana 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Arabidopsis thaliana ecotype Col-0 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine the enzyme is involved in the biosynthesis of thiamine diphosphate Arabidopsis thaliana ecotype Col-0 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.17 ? x * 71994, calculated from sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.1.99.17 thiC
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.17 iron-sulfur centre
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
4.1.99.17 Arabidopsis thaliana the THIC gene is negatively regulated by thiamin itself, regulation by conserved regions of mRNA that bind specific metabolites (riboswitches). As the thiamine riboswitch only responds to thiamin diphosphate but not thiamine, the down-regulation of THIC mRNA implies that the externally provided thiamine is converted to thiamine diphosphate inside the cell leading to the conformational change inducing mRNA instability down
4.1.99.17 Arabidopsis thaliana the transcript level in seedlings grown in 24 h of light is substantially higher than that in seedlings exposed to a single long day cycle (16-h light/8-h dark) up
4.1.99.17 Arabidopsis thaliana THIC transcript is not detectable two days after germination, but is readily detectable at day five up

General Information

EC Number General Information Comment Organism
4.1.99.17 malfunction knockdown mutant, if the thiC plants are not supplemented with thiamine, they eventually die. A concentration of 1.5 M thiamine is sufficient to allow growth of the seedlings, but these are chlorotic Arabidopsis thaliana
4.1.99.17 physiological function the enzyme is involved in the biosynthesis of thiamine diphosphate, the enzyme is essential for plant viability Arabidopsis thaliana