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Literature summary extracted from

  • Laing, W.A.; Bulley, S.; Wright, M.; Cooney, J.; Jensen, D.; Barraclough, D.; MacRae, E.
    A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis (2004), Proc. Natl. Acad. Sci. USA, 101, 16976-16981.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.B9 transformed into Escherichia coli DH5alpha cells Actinidia deliciosa
3.1.3.93 expression in Escherichia coli Actinidia deliciosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.B9 LiCl
-
 Arabidopsis thaliana
3.1.3.B9 Mg2+ above 0.2 mM Actinidia deliciosa
3.1.3.93 EDTA no activity is found in the presence of 0.2 mM EDTA without added Mg2+, native enzyme Actinidia deliciosa
3.1.3.93 LiCl inhibition of recombinant enzyme Actinidia deliciosa
3.1.3.93 Mg2+ above 2 mM, native enzyme Actinidia deliciosa
3.1.3.93 Mg2+
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.B9 0.022
-
 L-galactose 1-phosphate pH 7.0, 30°C, 4.8 mM MgCl2 Actinidia deliciosa
3.1.3.B9 0.041
-
 L-galactose 1-phosphate pH 7.0, 30°C, 1.8 mM MgCl2 Actinidia deliciosa
3.1.3.B9 0.15
-
 L-galactose 1-phosphate pH 7.0, 30°C Arabidopsis thaliana
3.1.3.93 0.01
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 1.8 mM Mg2+, native enzyme Actinidia deliciosa
3.1.3.93 0.022
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 4.8 mM Mg2+ Actinidia deliciosa
3.1.3.93 0.044
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, 2 mM Mg2+ Arabidopsis thaliana
3.1.3.93 0.15
-
 beta-L-galactose 1-phosphate pH 7.0, 30°C, recombinant enzyme Actinidia deliciosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.B9 Mg2+ the enzyme is completely dependent on Mg2+ for activity. Ka(Mg2+): 0.2 mM Actinidia deliciosa
3.1.3.93 Mg2+ absolutely dependent on Mg2+. K1 value: 0.016 mM Arabidopsis thaliana
3.1.3.93 Mg2+ completely dependent on Mg2+ for activity, Ka-value: 0.2 mM Actinidia deliciosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.B9 29121
-
 2 * 29121, calculated from sequence Arabidopsis thaliana
3.1.3.B9 65000
-
-
 Actinidia deliciosa
3.1.3.93 29121
-
 x * 29121, calculated from sequence Arabidopsis thaliana
3.1.3.93 29226
-
 2 * 29226, calculated from sequence Actinidia deliciosa
3.1.3.93 65000
-
 gel filtration, native enzyme Actinidia deliciosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.B9 L-galactose 1-phosphate + H2O Actinidia deliciosa
-
 L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O Arabidopsis thaliana the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O Arabidopsis thaliana Columbia-1 the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O Actinidia deliciosa A. Chev.
-
 L-galactose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.B9 Actinidia deliciosa Q5U789
-
-
3.1.3.B9 Actinidia deliciosa A. Chev. Q5U789
-
-
3.1.3.B9 Arabidopsis thaliana Q9M8S8
-
-
3.1.3.93 Actinidia deliciosa Q5U789
-
-
3.1.3.93 Arabidopsis thaliana Q9M8S8
-
-
3.1.3.93 Arabidopsis thaliana Columbia-1 Q9M8S8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.B9 partial Actinidia deliciosa
3.1.3.93
-
 Actinidia deliciosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.B9 fruit
-
 Arabidopsis thaliana
-
3.1.3.B9 fruit
-
 Actinidia deliciosa
-
3.1.3.93 fruit
-
 Actinidia deliciosa
-
3.1.3.93 shoot
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.B9 16.4
-
 pH 7.0, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.B9 L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
3.1.3.B9 L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
3.1.3.B9 additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana ?
-
 ?
3.1.3.B9 additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana Columbia-1 ?
-
 ?
3.1.3.93 beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Arabidopsis thaliana L-galactose + phosphate
-
 ?
3.1.3.93 beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Actinidia deliciosa L-galactose + phosphate
-
 ?
3.1.3.93 beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
3.1.3.93 beta-L-galactose 1-phosphate + H2O highly specific for beta-L-galactose 1-phosphate Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.B9 dimer 2 * 29121, calculated from sequence Arabidopsis thaliana
3.1.3.93 ? x * 29121, calculated from sequence Arabidopsis thaliana
3.1.3.93 dimer 2 * 29226, calculated from sequence Actinidia deliciosa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.B9 L-Gal-1-Pase
-
 Arabidopsis thaliana
3.1.3.B9 L-Gal-1-Pase
-
 Actinidia deliciosa
3.1.3.93 At3g02870
-
 Arabidopsis thaliana
3.1.3.93 IMPL2 gene name, ambiguous Actinidia deliciosa
3.1.3.93 L-Gal-1-P phosphatase
-
 Arabidopsis thaliana
3.1.3.93 L-Gal-1-P phosphatase
-
 Actinidia deliciosa
3.1.3.93 L-Gal-1-Pase
-
 Actinidia deliciosa
3.1.3.93 VTC4 gene name, ambiguous Arabidopsis thaliana
3.1.3.93 VTC4 gene name, ambiguous Actinidia deliciosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.B9 30
-
 assay at Actinidia deliciosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.B9 7
-
-
 Arabidopsis thaliana
3.1.3.B9 7
-
-
 Actinidia deliciosa
3.1.3.93 6.8 7 at 2 mM MgCl2 and 0.5 mM L-galactose 1-phosphate Arabidopsis thaliana
3.1.3.93 7
-
-
 Actinidia deliciosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.B9 6.2 7.5 pH 6.2: about 20% of maximal activity, pH 7.5: about 35% of maximal activity Actinidia deliciosa
3.1.3.93 6.5 7.8 pH 6.5: about 70% of maximal activity, pH 7.8: about 0% of maximal activity Actinidia deliciosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.B9 3.7
-
 LiCl pH 7.0, 30°C Arabidopsis thaliana
3.1.3.93 0.6
-
 Mg2+ pH 7.0, 30°C, inhibition of native enzyme Actinidia deliciosa
3.1.3.93 0.62
-
 Mg2+ pH 7.0, 30°C Arabidopsis thaliana
3.1.3.93 3.7
-
 LiCl pH 7.0, 30°C, inhibition of recombinant enzyme Actinidia deliciosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.B9 Arabidopsis thaliana calculated from sequence
-
 5.01
3.1.3.93 Arabidopsis thaliana calculated from sequence
-
 5.01