Literature summary extracted from

Kirby, J.; Nishimoto, M.; Park, J.G.; Withers, S.T.; Nowroozi, F.; Behrendt, D.; Rutledge, E.J.; Fortman, J.L.; Johnson, H.E.; Anderson, J.V.; Keasling, J.D.
Cloning of casbene and neocembrene synthases from Euphorbiaceae plants and expression in Saccharomyces cerevisiae (2010), Phytochemistry, 71, 1466-1473.

Application

EC Number	Application	Comment	Organism
4.2.3.8	synthesis	overexpression of truncated protein in Saccharomyces cerevisiae demonstrates casbene production but also high levels of nerolidol and farnesol and mevalonate	Euphorbia esula
4.2.3.8	synthesis	overexpression of truncated protein in Saccharomyces cerevisiae demonstrates casbene production up to 26 mg/l	Ricinus communis
4.2.3.8	synthesis	overexpression of truncated protein in Saccharomyces cerevisiae demonstrates casbene production up to 30 mg/l	Triadica sebifera
4.2.3.B23	synthesis	overexpression of truncated protein in Saccharomyces cerevisiae demonstrates casbene production up to 26 mg/l	Ricinus communis

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.3.8	Euphorbia esula	D3JYA2	-	-
4.2.3.8	Ricinus communis	B9RHX4	putative	-
4.2.3.8	Triadica sebifera	D3JYA1	-	-
4.2.3.B23	Ricinus communis	B9RHW5	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.3.8	geranylgeranyl diphosphate	-	Euphorbia esula	casbene + diphosphate	plus neocembrene, approximately 3% of the casbene product	?
4.2.3.8	geranylgeranyl diphosphate	-	Triadica sebifera	casbene + diphosphate	plus neocembrene, approximately 3% of the casbene product	?
4.2.3.8	geranylgeranyl diphosphate	-	Ricinus communis	casbene + diphosphate	plus neocembrene, approximately 3% of the casbene product	?
4.2.3.B23	geranylgeranyl diphosphate	-	Ricinus communis	neocembrene + diphosphate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.3.8	TPS2	-	Euphorbia esula
4.2.3.8	TPS2	-	Triadica sebifera
4.2.3.8	TPS2	-	Ricinus communis
4.2.3.B23	TPS2	-	Ricinus communis

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Release 2023.1 (January 2023)