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Literature summary extracted from

  • Hoshino, T.; Nakano, C.; Ootsuka, T.; Shinohara, Y.; Hara, T.
    Substrate specificity of Rv3378c, an enzyme from Mycobacterium tuberculosis, and the inhibitory activity of the bicyclic diterpenoids against macrophage phagocytosis (2011), Org. Biomol. Chem., 9, 2156-2165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.153 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
4.2.3.B30 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B31 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B32 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B33 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B34 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B35 expression in Escherichia coli Mycobacterium tuberculosis
4.2.3.B36 expression in Escherichia coli Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.153 Mg2+ essential for the enzymatic activities Mycobacterium tuberculosis
4.2.3.B30 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B31 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B32 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B33 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B34 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B35 Mg2+ essentially required Mycobacterium tuberculosis
4.2.3.B36 Mg2+ essentially required Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.153 Mycobacterium tuberculosis
-
-
-
2.5.1.153 Mycobacterium tuberculosis H37Rv
-
-
-
4.2.3.B30 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B30 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B31 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B31 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B32 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B32 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B33 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B33 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B34 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B34 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B35 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B35 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-
4.2.3.B36 Mycobacterium tuberculosis P9WJ61 putative
-
4.2.3.B36 Mycobacterium tuberculosis H37Rv P9WJ61 putative
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.B30 (+)-copalyl diphosphate + H2O = (+)-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B31 (+)-copalyl diphosphate + H2O = manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B32 (+)-copalyl diphosphate + H2O = 13-epi-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B33 (-)-ent-copalyl diphosphate + H2O = (-)-ent-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 22% (-)-ent-copalol, 8% ent-13-epi-manool plus 42% ent-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B34 (-)-ent-copalyl diphosphate + H2O = ent-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 22% (-)-ent-copalol, 8% ent-13-epi-manool plus 42% ent-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B35 syn-copalyl diphosphate + H2O = syn-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 16% syn-copalol, 59% (13S)-vitexifolin A plus 3% (13R)-vitexifolin A. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a
4.2.3.B36 syn-copalyl diphosphate + H2O = (13S)-vitexifolin A + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 16% syn-copalol, 59% (13S)-vitexifolin A plus 3% (13R)-vitexifolin A. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation Mycobacterium tuberculosis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.153 2 tuberculosinyl diphosphate + 2 H2O
-
 Mycobacterium tuberculosis (13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3 ?
2.5.1.153 2 tuberculosinyl diphosphate + 2 H2O
-
 Mycobacterium tuberculosis H37Rv (13R)-isotuberculosinol + (13S)-isotuberculosinol + 2 diphosphate the product ratio of (13R)-isotuberculosinol and (13S)-isotuberculosinol is 1:3 ?
2.5.1.153 copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis (+)-copalol + manool + 13-epi-manool + diphosphate 40% (+)-copalol, 12% manool and 15% 13-epi-manool ?
2.5.1.153 copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv (+)-copalol + manool + 13-epi-manool + diphosphate 40% (+)-copalol, 12% manool and 15% 13-epi-manool ?
2.5.1.153 ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis (-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate 22% (-)-ent copalol, 8% ent-13-epi-mancol, and 42% ent-manool ?
2.5.1.153 ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv (-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate 22% (-)-ent copalol, 8% ent-13-epi-mancol, and 42% ent-manool ?
2.5.1.153 additional information no activity with terpentedienyl diphosphate, geranylgeranyl diphosphate, farnesyl diphosphate, and geranyl diphosphate Mycobacterium tuberculosis ?
-
 ?
2.5.1.153 additional information no activity with terpentedienyl diphosphate, geranylgeranyl diphosphate, farnesyl diphosphate, and geranyl diphosphate Mycobacterium tuberculosis H37Rv ?
-
 ?
2.5.1.153 syn-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate 16% syn-copalol, 3% (13R)-vitrxifolin and 59% (13S)-vitexifolin ?
2.5.1.153 syn-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate 16% syn-copalol, 3% (13R)-vitrxifolin and 59% (13S)-vitexifolin ?
2.5.1.153 tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis tuberculosinol + diphosphate
-
 ?
4.2.3.B30 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis (+)-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B30 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv (+)-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B31 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B31 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B32 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis 13-epi-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B32 (+)-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv 13-epi-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B33 (-)-ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis (-)-ent-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B33 (-)-ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv (-)-ent-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B34 (-)-ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis ent-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B34 (-)-ent-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis H37Rv ent-manool + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool ?
4.2.3.B35 syn-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis syn-copalol + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 16% syn-copalol, 59% (13S)-vitexifolin A plus 3% (13R)-vitexifolin A ?
4.2.3.B36 syn-copalyl diphosphate + H2O
-
 Mycobacterium tuberculosis (13S)-vitexifolin A + diphosphate products of Mycobacterium tuberculosis gene product Rv3378c are 16% syn-copalol, 59% (13S)-vitexifolin A plus 3% (13R)-vitexifolin A ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.153 Rv3378c protein
-
 Mycobacterium tuberculosis
4.2.3.B30 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B31 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B32 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B33 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B34 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B35 Rv3378c
-
 Mycobacterium tuberculosis
4.2.3.B36 Rv3378c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.153 45
-
-
 Mycobacterium tuberculosis
4.2.3.B30 45
-
-
 Mycobacterium tuberculosis
4.2.3.B31 45
-
-
 Mycobacterium tuberculosis
4.2.3.B32 45
-
-
 Mycobacterium tuberculosis
4.2.3.B33 45
-
-
 Mycobacterium tuberculosis
4.2.3.B34 45
-
-
 Mycobacterium tuberculosis
4.2.3.B35 45
-
-
 Mycobacterium tuberculosis
4.2.3.B36 45
-
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.153 7
-
-
 Mycobacterium tuberculosis
4.2.3.B30 7
-
-
 Mycobacterium tuberculosis
4.2.3.B31 7
-
-
 Mycobacterium tuberculosis
4.2.3.B32 7
-
-
 Mycobacterium tuberculosis
4.2.3.B33 7
-
-
 Mycobacterium tuberculosis
4.2.3.B34 7
-
-
 Mycobacterium tuberculosis
4.2.3.B35 7
-
-
 Mycobacterium tuberculosis
4.2.3.B36 7
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.153 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B30 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B31 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B32 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B33 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B34 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B35 5.5 9
-
 Mycobacterium tuberculosis
4.2.3.B36 5.5 9
-
 Mycobacterium tuberculosis