BRENDA - Enzyme Database

5'-Nucleotidases and their new roles in NAD+ and phosphate metabolism

Bogan, K.; Brenner, C.; New J. Chem. 34, 845-853 (2010)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
2,3-bisphosphoglycerate
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
ATP
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
dATP
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
Diadenosine tetraphosphate
activates cytosolic 5'-nucleotidase II by increase binding and dimer formation, interface binding structure modeling
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.5
gene NT5E at 6q14-q21 encodes CD73
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
alpha/beta-methylene-ADP
a specific CD73 inhibitor
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
additional information
-
additional information
complex allosteric regulation of cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
1.2
-
5'-UMP
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
3.1.3.5
2.3
-
5'-CMP
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cell surface
CD73
Homo sapiens
9986
-
3.1.3.5
cytosol
-
Homo sapiens
5829
-
3.1.3.5
extracellular
-
Corynebacterium glutamicum
-
-
3.1.3.5
extracellular
cell surface
Homo sapiens
-
-
3.1.3.5
intracellular
-
Homo sapiens
5622
-
3.1.3.5
membrane
membrane-bound 5'-nucleotidases are ectoenzymes
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
71000
-
homodimeric CD73
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
5'-AMP + H2O
Homo sapiens
-
adenosine + phosphate
-
-
?
3.1.3.5
5'-CMP + H2O
Homo sapiens
-
cytidine + phosphate
-
-
?
3.1.3.5
5'-CMP + H2O
Saccharomyces cerevisiae
Sdt1
cytidine + phosphate
-
-
?
3.1.3.5
5'-dCMP + H2O
Homo sapiens
-
2'-deoxycytidine + phosphate
-
-
?
3.1.3.5
5'-dGMP + H2O
Homo sapiens
-
deoxyguanosine + phosphate
-
-
?
3.1.3.5
5'-dIMP + H2O
Homo sapiens
-
deoxyinosine + phosphate
-
-
?
3.1.3.5
5'-dTMP + H2O
Homo sapiens
-
deoxythymidine + phosphate
-
-
?
3.1.3.5
5'-dUMP + H2O
Homo sapiens
-
deoxyuridine + phosphate
-
-
?
3.1.3.5
5'-GMP + H2O
Homo sapiens
-
guanosine + phosphate
-
-
?
3.1.3.5
5'-IMP + H2O
Homo sapiens
-
inosine + phosphate
-
-
?
3.1.3.5
5'-IMP + H2O
Saccharomyces cerevisiae
Isn1
inosine + phosphate
-
-
?
3.1.3.5
5'-UMP + H2O
Homo sapiens
-
uridine + phosphate
-
-
?
3.1.3.5
5'-UMP + H2O
Saccharomyces cerevisiae
Sdt1
uridine + phosphate
-
-
?
3.1.3.5
5'-XMP + H2O
Homo sapiens
-
xanthosine + phosphate
-
-
?
3.1.3.5
additional information
Saccharomyces cerevisiae
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
?
-
-
-
3.1.3.5
additional information
Homo sapiens
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Corynebacterium glutamicum
-
-
-
3.1.3.5
Homo sapiens
-
gene NT5E at 6q14-q21 encodes CD73
-
3.1.3.5
Saccharomyces cerevisiae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
B-lymphocyte
CD73 is a lymphocyte surface antigen
Homo sapiens
-
3.1.3.5
dendritic cell
CD73
Homo sapiens
-
3.1.3.5
forestomach
CD73
Homo sapiens
-
3.1.3.5
kidney
CD73
Homo sapiens
-
3.1.3.5
additional information
the enzyme is ubiquitously expressed among cell types. Quantitative real time-PCR for determination of CD73 distribution. CD73 is widely expressed in vertebrate tissues and is prominently expressed on B and T cells and certain types of dendritic cells. It shows variable expression among cell types within other tissues including skeletal muscle, thymus, spleen, kidney and forestomach
Homo sapiens
-
3.1.3.5
skeletal muscle
CD73
Homo sapiens
-
3.1.3.5
spleen
CD73
Homo sapiens
-
3.1.3.5
T-lymphocyte
CD73 is a lymphocyte surface antigen
Homo sapiens
-
3.1.3.5
thymus
CD73
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
16600
-
substrate IMP, 5'-nucleotidase Isn1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
2'-chloro-2'-deoxyAMP + H2O
cNII
716355
Homo sapiens
2'-chloro-2'-deoxyadenosine + phosphate
-
-
-
?
3.1.3.5
3'-azido-3'deoxy-dTMP + H2O
cNII
716355
Homo sapiens
3'-azido-3'-deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-AMP + H2O
-
716355
Homo sapiens
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-AMP + H2O
CD73
716355
Homo sapiens
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
-
716355
Homo sapiens
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
Sdt1
716355
Saccharomyces cerevisiae
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
CD73
716355
Homo sapiens
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
-
716355
Homo sapiens
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
CD73
716355
Homo sapiens
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dGMP + H2O
-
716355
Homo sapiens
deoxyguanosine + phosphate
-
-
-
?
3.1.3.5
5'-dGMP + H2O
cNII
716355
Homo sapiens
deoxyguanosine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
-
716355
Homo sapiens
deoxyinosine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
cNII
716355
Homo sapiens
deoxyinosine + phosphate
-
-
-
?
3.1.3.5
5'-dTMP + H2O
-
716355
Homo sapiens
deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-dTMP + H2O
CD73
716355
Homo sapiens
deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-dUMP + H2O
-
716355
Homo sapiens
deoxyuridine + phosphate
-
-
-
?
3.1.3.5
5'-dUMP + H2O
CD73
716355
Homo sapiens
deoxyuridine + phosphate
-
-
-
?
3.1.3.5
5'-GMP + H2O
-
716355
Homo sapiens
guanosine + phosphate
-
-
-
?
3.1.3.5
5'-GMP + H2O
cNII, CD73
716355
Homo sapiens
guanosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
-
716355
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
Isn1
716355
Saccharomyces cerevisiae
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
cNII, CD73
716355
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
-
716355
Homo sapiens
uridine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
Sdt1
716355
Saccharomyces cerevisiae
uridine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
CD73
716355
Homo sapiens
uridine + phosphate
-
-
-
?
3.1.3.5
5'-XMP + H2O
-
716355
Homo sapiens
xanthosine + phosphate
-
-
-
?
3.1.3.5
5'-XMP + H2O
cNII, CD73
716355
Homo sapiens
xanthosine + phosphate
-
-
-
?
3.1.3.5
5-fluoro-dUMP + H2O
cNII
716355
Homo sapiens
5-fluoro-uridine + phosphate
-
-
-
?
3.1.3.5
additional information
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
716355
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.5
additional information
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
716355
Homo sapiens
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
homodimer
CD73
Homo sapiens
3.1.3.5
tetramer
cytosolic 5'-nucleotidase II, cNII, is a tetramer, formed from two homodimers
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
2,3-bisphosphoglycerate
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
ATP
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
dATP
activates cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
Diadenosine tetraphosphate
activates cytosolic 5'-nucleotidase II by increase binding and dimer formation, interface binding structure modeling
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
gene NT5E at 6q14-q21 encodes CD73
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.5
alpha/beta-methylene-ADP
a specific CD73 inhibitor
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
additional information
-
additional information
complex allosteric regulation of cytosolic 5'-nucleotidase II
Homo sapiens
3.1.3.5
1.2
-
5'-UMP
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
3.1.3.5
2.3
-
5'-CMP
5'-nucleotidase Sdt1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
cell surface
CD73
Homo sapiens
9986
-
3.1.3.5
cytosol
-
Homo sapiens
5829
-
3.1.3.5
extracellular
-
Corynebacterium glutamicum
-
-
3.1.3.5
extracellular
cell surface
Homo sapiens
-
-
3.1.3.5
intracellular
-
Homo sapiens
5622
-
3.1.3.5
membrane
membrane-bound 5'-nucleotidases are ectoenzymes
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
71000
-
homodimeric CD73
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
5'-AMP + H2O
Homo sapiens
-
adenosine + phosphate
-
-
?
3.1.3.5
5'-CMP + H2O
Homo sapiens
-
cytidine + phosphate
-
-
?
3.1.3.5
5'-CMP + H2O
Saccharomyces cerevisiae
Sdt1
cytidine + phosphate
-
-
?
3.1.3.5
5'-dCMP + H2O
Homo sapiens
-
2'-deoxycytidine + phosphate
-
-
?
3.1.3.5
5'-dGMP + H2O
Homo sapiens
-
deoxyguanosine + phosphate
-
-
?
3.1.3.5
5'-dIMP + H2O
Homo sapiens
-
deoxyinosine + phosphate
-
-
?
3.1.3.5
5'-dTMP + H2O
Homo sapiens
-
deoxythymidine + phosphate
-
-
?
3.1.3.5
5'-dUMP + H2O
Homo sapiens
-
deoxyuridine + phosphate
-
-
?
3.1.3.5
5'-GMP + H2O
Homo sapiens
-
guanosine + phosphate
-
-
?
3.1.3.5
5'-IMP + H2O
Homo sapiens
-
inosine + phosphate
-
-
?
3.1.3.5
5'-IMP + H2O
Saccharomyces cerevisiae
Isn1
inosine + phosphate
-
-
?
3.1.3.5
5'-UMP + H2O
Homo sapiens
-
uridine + phosphate
-
-
?
3.1.3.5
5'-UMP + H2O
Saccharomyces cerevisiae
Sdt1
uridine + phosphate
-
-
?
3.1.3.5
5'-XMP + H2O
Homo sapiens
-
xanthosine + phosphate
-
-
?
3.1.3.5
additional information
Saccharomyces cerevisiae
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
?
-
-
-
3.1.3.5
additional information
Homo sapiens
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
B-lymphocyte
CD73 is a lymphocyte surface antigen
Homo sapiens
-
3.1.3.5
dendritic cell
CD73
Homo sapiens
-
3.1.3.5
forestomach
CD73
Homo sapiens
-
3.1.3.5
kidney
CD73
Homo sapiens
-
3.1.3.5
additional information
the enzyme is ubiquitously expressed among cell types. Quantitative real time-PCR for determination of CD73 distribution. CD73 is widely expressed in vertebrate tissues and is prominently expressed on B and T cells and certain types of dendritic cells. It shows variable expression among cell types within other tissues including skeletal muscle, thymus, spleen, kidney and forestomach
Homo sapiens
-
3.1.3.5
skeletal muscle
CD73
Homo sapiens
-
3.1.3.5
spleen
CD73
Homo sapiens
-
3.1.3.5
T-lymphocyte
CD73 is a lymphocyte surface antigen
Homo sapiens
-
3.1.3.5
thymus
CD73
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
16600
-
substrate IMP, 5'-nucleotidase Isn1, pH not specified in the publication, temperature not specified in the publication
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
2'-chloro-2'-deoxyAMP + H2O
cNII
716355
Homo sapiens
2'-chloro-2'-deoxyadenosine + phosphate
-
-
-
?
3.1.3.5
3'-azido-3'deoxy-dTMP + H2O
cNII
716355
Homo sapiens
3'-azido-3'-deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-AMP + H2O
-
716355
Homo sapiens
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-AMP + H2O
CD73
716355
Homo sapiens
adenosine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
-
716355
Homo sapiens
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
Sdt1
716355
Saccharomyces cerevisiae
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-CMP + H2O
CD73
716355
Homo sapiens
cytidine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
-
716355
Homo sapiens
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dCMP + H2O
CD73
716355
Homo sapiens
2'-deoxycytidine + phosphate
-
-
-
?
3.1.3.5
5'-dGMP + H2O
-
716355
Homo sapiens
deoxyguanosine + phosphate
-
-
-
?
3.1.3.5
5'-dGMP + H2O
cNII
716355
Homo sapiens
deoxyguanosine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
-
716355
Homo sapiens
deoxyinosine + phosphate
-
-
-
?
3.1.3.5
5'-dIMP + H2O
cNII
716355
Homo sapiens
deoxyinosine + phosphate
-
-
-
?
3.1.3.5
5'-dTMP + H2O
-
716355
Homo sapiens
deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-dTMP + H2O
CD73
716355
Homo sapiens
deoxythymidine + phosphate
-
-
-
?
3.1.3.5
5'-dUMP + H2O
-
716355
Homo sapiens
deoxyuridine + phosphate
-
-
-
?
3.1.3.5
5'-dUMP + H2O
CD73
716355
Homo sapiens
deoxyuridine + phosphate
-
-
-
?
3.1.3.5
5'-GMP + H2O
-
716355
Homo sapiens
guanosine + phosphate
-
-
-
?
3.1.3.5
5'-GMP + H2O
cNII, CD73
716355
Homo sapiens
guanosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
-
716355
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
Isn1
716355
Saccharomyces cerevisiae
inosine + phosphate
-
-
-
?
3.1.3.5
5'-IMP + H2O
cNII, CD73
716355
Homo sapiens
inosine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
-
716355
Homo sapiens
uridine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
Sdt1
716355
Saccharomyces cerevisiae
uridine + phosphate
-
-
-
?
3.1.3.5
5'-UMP + H2O
CD73
716355
Homo sapiens
uridine + phosphate
-
-
-
?
3.1.3.5
5'-XMP + H2O
-
716355
Homo sapiens
xanthosine + phosphate
-
-
-
?
3.1.3.5
5'-XMP + H2O
cNII, CD73
716355
Homo sapiens
xanthosine + phosphate
-
-
-
?
3.1.3.5
5-fluoro-dUMP + H2O
cNII
716355
Homo sapiens
5-fluoro-uridine + phosphate
-
-
-
?
3.1.3.5
additional information
Sdt1 is highly specific for 5'-UMP and 5'-CMP, and shows negligible activity on purine nucleoside monophosphates, 3'-monophosphates or nucleoside triphosphates
716355
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.5
additional information
Usha, Pho5, Sdt1 and Phm8 are phosphate starvation-induced 5'-nucleotidases with diverse substrate specificities that liberate phosphate under phosphate starvation conditions
716355
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
homodimer
CD73
Homo sapiens
3.1.3.5
tetramer
cytosolic 5'-nucleotidase II, cNII, is a tetramer, formed from two homodimers
Homo sapiens
Expression
EC Number
Organism
Commentary
Expression
3.1.3.5
Corynebacterium glutamicum
5'-nucleotidase UshA is induced when phosphate is limiting
up
3.1.3.5
Homo sapiens
CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine. Though CD73 is mainly regulated transcriptionally, cross linking of CD38, a cell surface glycoprotein with cyclic ADP-ribose hydrolase activity, causes upregulation of CD73 via export of protein to the cell surface that is independent of new transcription
up
3.1.3.5
Saccharomyces cerevisiae
5'-nucleotidases Sdt1 and Phm8 are induced at the transcript level by phosphate starvation
up
General Information
EC Number
General Information
Commentary
Organism
3.1.3.5
malfunction
cytosolic 5'-nucleotidase cN-II is implicated in drug resistance in patients and dephosphorylates 5-fluoro-dUMP, 3'-azido-3-dTMP, 2'-chloro-2'-deoxyAMP, and related compounds, which represent the monophosphorylated forms of nucleoside prodrugs
Homo sapiens
3.1.3.5
metabolism
5'-nucleotidases are involved in the purine and pyrimidine nucleotide metabolic pathways, overview. CD73 enzymatic activity is implicated in two major extracellular metabolic pathways with distinct physiological roles, extracellular adenosine nucleotide and NAD+ metabolism
Homo sapiens
3.1.3.5
additional information
CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine
Homo sapiens
3.1.3.5
physiological function
5'-nucleotidase UshA is required for growth when nucleotides are provided as the sole source of phosphate, a condition that is likely relevant to bacterial growth in the wild
Corynebacterium glutamicum
3.1.3.5
physiological function
5'-nucleotidase activities regulate the quantity of nucleotides generated from both de novo and salvage pathways, overview. 5'-Nucleotidase designates a set of enzymes, which catalyze the hydrolysis of ribonucleoside and deoxyribonucleoside monophosphates into the corresponding nucleosides plus phosphate. 5'-Nucleotidases are classified according to subcellular localization, nucleobase specificity and their ability to hydrolyze deoxynucleoside monophosphate substrates. Membrane-bound 5'-nucleotidases are ectoenzymes principally involved in salvage of extracellular nucleosides, and often display a preference toward adenosine monophosphate, thereby modulating signal transduction cascades involving purinergic receptors. Cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily of enzymes, which are two-domain proteins containing a modified Rossman fold as the core and a variable cap structure. Extracellular and intracellular 5'-nucleotidase activities participate in purine and pyrimidine salvage to support balanced synthesis of nucleotides, which is critical for maintaining high fidelity DNA replication. The major physiological roles for CD73 in extracellular adenosine metabolism are severalfold, overview. cNII is subject to complex allosteric regulation
Homo sapiens
3.1.3.5
physiological function
ISN1 is responsible for virtually all IMP 5'-nucleotidase activity in budding yeast cells and is required for cells to release inosine into the culture medium
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.5
malfunction
cytosolic 5'-nucleotidase cN-II is implicated in drug resistance in patients and dephosphorylates 5-fluoro-dUMP, 3'-azido-3-dTMP, 2'-chloro-2'-deoxyAMP, and related compounds, which represent the monophosphorylated forms of nucleoside prodrugs
Homo sapiens
3.1.3.5
metabolism
5'-nucleotidases are involved in the purine and pyrimidine nucleotide metabolic pathways, overview. CD73 enzymatic activity is implicated in two major extracellular metabolic pathways with distinct physiological roles, extracellular adenosine nucleotide and NAD+ metabolism
Homo sapiens
3.1.3.5
additional information
CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine
Homo sapiens
3.1.3.5
physiological function
5'-nucleotidase UshA is required for growth when nucleotides are provided as the sole source of phosphate, a condition that is likely relevant to bacterial growth in the wild
Corynebacterium glutamicum
3.1.3.5
physiological function
5'-nucleotidase activities regulate the quantity of nucleotides generated from both de novo and salvage pathways, overview. 5'-Nucleotidase designates a set of enzymes, which catalyze the hydrolysis of ribonucleoside and deoxyribonucleoside monophosphates into the corresponding nucleosides plus phosphate. 5'-Nucleotidases are classified according to subcellular localization, nucleobase specificity and their ability to hydrolyze deoxynucleoside monophosphate substrates. Membrane-bound 5'-nucleotidases are ectoenzymes principally involved in salvage of extracellular nucleosides, and often display a preference toward adenosine monophosphate, thereby modulating signal transduction cascades involving purinergic receptors. Cytosolic 5'-nucleotidases are members of the haloacid dehalogenase superfamily of enzymes, which are two-domain proteins containing a modified Rossman fold as the core and a variable cap structure. Extracellular and intracellular 5'-nucleotidase activities participate in purine and pyrimidine salvage to support balanced synthesis of nucleotides, which is critical for maintaining high fidelity DNA replication. The major physiological roles for CD73 in extracellular adenosine metabolism are severalfold, overview. cNII is subject to complex allosteric regulation
Homo sapiens
3.1.3.5
physiological function
ISN1 is responsible for virtually all IMP 5'-nucleotidase activity in budding yeast cells and is required for cells to release inosine into the culture medium
Saccharomyces cerevisiae
Expression (protein specific)
EC Number
Organism
Commentary
Expression
3.1.3.5
Corynebacterium glutamicum
5'-nucleotidase UshA is induced when phosphate is limiting
up
3.1.3.5
Homo sapiens
CD73 is highly induced by ischemia and hypoxia, and increased expression in cardiac ischemia is mediated by a positive feedback loop involving adenosine. Though CD73 is mainly regulated transcriptionally, cross linking of CD38, a cell surface glycoprotein with cyclic ADP-ribose hydrolase activity, causes upregulation of CD73 via export of protein to the cell surface that is independent of new transcription
up
3.1.3.5
Saccharomyces cerevisiae
5'-nucleotidases Sdt1 and Phm8 are induced at the transcript level by phosphate starvation
up