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Literature summary extracted from

  • Duane, W.; Hastings, J.W.
    Flavin mononucleotide reductase of luminous bacteria (1975), Mol. Cell. Biochem., 6, 53-64.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.42 antimycin A 0.05 mM, 50% inhibition Vibrio harveyi
1.5.1.42 additional information no inhibition by 0.01 mM 2,4-dinitrophenol, 0.2 mM dicoumarol, 0.1 mM rotenone Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.42 23000
-
 gel filtration Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.42 FMN + NADH + H+ Vibrio harveyi
-
 FMNH2 + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.42 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.42
-
 Vibrio harveyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.42 additional information
-
 purity was too low to permit estimation of specific activity Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.42 FAD + NADH + H+ activity with FADH2 and NAD+ is 5% of the activity with FMNH2 and NAD+ Vibrio harveyi FADH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Vibrio harveyi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+ activity with NADP+ and FMNH2 is only 10% of the activity with FMNH2 and NAD+ Vibrio harveyi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADPH + H+ activity with NADP+ and FMNH2 is only 10% of the activity with FMNH2 and NAD+ Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.42 additional information activity with FAD and NADPH is 0.5% of the activity with FMNH2 and NAD+ Vibrio harveyi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.42 NADH:FMN oxidoreductase (flavin reductase)
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.42 20
-
 assay at Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.42 7
-
 assay at Vibrio harveyi
1.5.1.42 8.5
-
-
 Vibrio harveyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.42 5 10 bimodal, pH 5: 90% of maximal activity, pH 6.5: about 50% of maximal activity, pH 10: about 70% of maximal activity Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.42 NADH activity with NADPH and FMNH2 is only 10% of the activity with FMNH2 and NAD+ Vibrio harveyi
1.5.1.42 NADPH activity with NADPH and FMNH2 is only 10% of the activity with FMNH2 and NAD+ Vibrio harveyi