Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Verhagen, M.; Adams, M.
    Fe-only hydrogenase from Thermotoga maritima (2001), Methods Enzymol., 331, 216-226.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.4 Fe2+ the holoprotein contains 31.1 mol iron per mol enzyme Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.4 18025
-
 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
1.12.1.4 19000
-
 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
1.12.1.4 68000
-
 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
1.12.1.4 68676
-
 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
1.12.1.4 72248
-
 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
1.12.1.4 73000
-
 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
1.12.1.4 158900
-
 gel filtration Thermotoga maritima
1.12.1.4 160000
-
 calculated from amino acid sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.4 NADH + H+ + reduced ferredoxin Thermotoga maritima
-
 H2 + NAD+ + oxidized ferredoxin
-
 ?
1.12.1.4 NADH + H+ + reduced ferredoxin Thermotoga maritima DSM 3109
-
 H2 + NAD+ + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.4 Thermotoga maritima
-
-
-
1.12.1.4 Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.4 DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.4 NADH + H+ + reduced ferredoxin
-
 Thermotoga maritima H2 + NAD+ + oxidized ferredoxin
-
 ?
1.12.1.4 NADH + H+ + reduced ferredoxin
-
 Thermotoga maritima DSM 3109 H2 + NAD+ + oxidized ferredoxin
-
 ?
1.12.1.4 sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
 Thermotoga maritima H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
 ?
1.12.1.4 sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
 Thermotoga maritima DSM 3109 H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
 ?
1.12.1.4 sodium dithionite + H+ + reduced methyl viologen
-
 Thermotoga maritima H2 + oxidized methyl viologen + ?
-
 ?
1.12.1.4 sodium dithionite + H+ + reduced methyl viologen
-
 Thermotoga maritima DSM 3109 H2 + oxidized methyl viologen + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.4 heterotrimer 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
1.12.1.4 heterotrimer 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.12.1.4 Fe-only hydrogenase
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.4 flavin
-
 Thermotoga maritima
1.12.1.4 iron-sulfur centre the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster Thermotoga maritima