Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, J.; Dong, S.; Song, J.; Li, C.; Chen, X.; Xie, B.; Zhang, Y.
    Purification and characterization of a bifunctional alginate lyase from Pseudoalteromonas sp. SM0524 (2011), Mar. Drugs, 9, 109-123.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.2.3 synthesis aly-SJ02 may be a good tool to produce dimers and trimers from alginate Pseudoalteromonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Cu2+ slightly inhibiting Pseudoalteromonas sp.
4.2.2.3 EDTA 1 mM, 48% of initial activity; 51.7% inhibition at 1 mM Pseudoalteromonas sp.
4.2.2.3 Sn2+ slightly inhibiting Pseudoalteromonas sp.
4.2.2.11 EDTA 1 mM, 48% of initial activity Pseudoalteromonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information KM-value for alpha-L-guluronosyl linkage in alginate, 1.086 mg/ml, for poly(alpha-L-guluronic acid), 2.751 mg/ml at pH 8.5, 50°C Pseudoalteromonas sp.
4.2.2.11 additional information
-
 additional information KM-value for alpha-L-guluronosyl linkage in alginate, 1.086 mg/ml, for poly(alpha-L-guluronic acid), 0.465 mg/ml at pH 8.5, 50°C Pseudoalteromonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.3 extracellular
-
 Pseudoalteromonas sp.
-
-
4.2.2.3 extracellular aly-SJ02 is secreted Pseudoalteromonas sp.
-
-
4.2.2.11 extracellular
-
 Pseudoalteromonas sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ba2+ activates Pseudoalteromonas sp.
4.2.2.3 Ba2+ 1mM, 143% of initial activity Pseudoalteromonas sp.
4.2.2.3 Ca2+ activates Pseudoalteromonas sp.
4.2.2.3 Ca2+ 1mM, 136% of initial activity Pseudoalteromonas sp.
4.2.2.3 Co2+ activates Pseudoalteromonas sp.
4.2.2.3 Co2+ 1mM, 123% of initial activity Pseudoalteromonas sp.
4.2.2.3 K+ activates Pseudoalteromonas sp.
4.2.2.3 K+ 1mM, 117% of initial activity Pseudoalteromonas sp.
4.2.2.3 Mg2+ activates Pseudoalteromonas sp.
4.2.2.3 Mg2+ 1mM, 126% of initial activity Pseudoalteromonas sp.
4.2.2.3 Mn2+ 1mM, 116% of initial activity Pseudoalteromonas sp.
4.2.2.3 additional information Zn2+ has no effect on aly-SJ02 activity Pseudoalteromonas sp.
4.2.2.3 Na+ activates Pseudoalteromonas sp.
4.2.2.3 Na+ 1mM, 143% of initial activity Pseudoalteromonas sp.
4.2.2.3 NaCl aly-SJ02 shows the highest activity in 0.2 M NaCl, and retains more than 75% activity in 1 M NaCl, exhibiting salt-tolerance ability Pseudoalteromonas sp.
4.2.2.3 Ni2+ activates Pseudoalteromonas sp.
4.2.2.3 Ni2+ 1mM, 110% of initial activity Pseudoalteromonas sp.
4.2.2.3 Sr2+ activates Pseudoalteromonas sp.
4.2.2.3 Sr2+ 1mM, 124% of initial activity Pseudoalteromonas sp.
4.2.2.11 Ba2+ 1 mM, 143% of initial activity Pseudoalteromonas sp.
4.2.2.11 Ca2+ 1 mM, 136% of initial activity Pseudoalteromonas sp.
4.2.2.11 Co2+ 1 mM, 123% of initial activity Pseudoalteromonas sp.
4.2.2.11 K+ 1 mM, 117% of initial activity Pseudoalteromonas sp.
4.2.2.11 Mg2+ 1 mM, 126% of initial activity Pseudoalteromonas sp.
4.2.2.11 Mn2+ 1 mM, 116% of initial activity Pseudoalteromonas sp.
4.2.2.11 Na+ 1 mM, 143% of initial activity Pseudoalteromonas sp.
4.2.2.11 Ni2+ 1 mM, 110% of initial activity Pseudoalteromonas sp.
4.2.2.11 Sr2+ 1 mM, 124% of initial activity Pseudoalteromonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 32000
-
 x * 32000, SDS-PAGE Pseudoalteromonas sp.
4.2.2.11 32000
-
 x * 32000, SDS-PAGE Pseudoalteromonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 additional information Pseudoalteromonas sp. alginate is a linear hetero-polyuronic acid composed of 1,4 linked alpha-L-guluronic acid (G) and beta-D-mannuronic acid. Alginate lyase aly-SJ02 is bifunctional. Aly-SJ02 shows activities toward both polyG (alpha-L-guluronic acid), activity of EC 4.2.2.11, and polyM (beta-D-mannuronic acid). Aly-SJ02 mainly releases dimers and trimers from polyM and alginate, and trimers and tetramers from polyG ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudoalteromonas sp.
-
 bifunctional enzyme, actitivies against poly(beta-D-mannuronate), EC 4.2.2.3, and poly(alpha-L-guluronate), EC 4.2.2.11
-
4.2.2.3 Pseudoalteromonas sp.
-
 from marine rotten kelp from a kelp culture field at the seashore of Yantai, China
-
4.2.2.11 Pseudoalteromonas sp.
-
 bifunctional enzyme, activities against poly(beta-D-mannuronate), EC 4.2.2.3, and poly(alpha-L-guluronate), EC 4.2.2.11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3
-
 Pseudoalteromonas sp.
4.2.2.3 native, secreted aly-SJ02 73.4fold by ultrafiltration, anion exchange chromatography, and gel filtration Pseudoalteromonas sp.
4.2.2.11
-
 Pseudoalteromonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 4803
-
 pH 8.5, 50°C Pseudoalteromonas sp.
4.2.2.3 4803
-
 purified enzyme, pH 8.5, 50°C Pseudoalteromonas sp.
4.2.2.11 4803
-
 pH 8.5, 50°C Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alpha-L-mannuronyl linkage in alginate
-
 Pseudoalteromonas sp. ? enzyme shows activities toward both polyG, i.e.alpha-L-guluronic acid, and polyM, i.e. beta-D-mannuronic acid ?
4.2.2.3 additional information alginate is a linear hetero-polyuronic acid composed of 1,4 linked alpha-L-guluronic acid (G) and beta-D-mannuronic acid. Alginate lyase aly-SJ02 is bifunctional. Aly-SJ02 shows activities toward both polyG (alpha-L-guluronic acid), activity of EC 4.2.2.11, and polyM (beta-D-mannuronic acid). Aly-SJ02 mainly releases dimers and trimers from polyM and alginate, and trimers and tetramers from polyG Pseudoalteromonas sp. ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronate)
-
 Pseudoalteromonas sp. ?
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate
-
 Pseudoalteromonas sp. ? enzyme shows activities toward both polyG, i.e. poly-alpha-(1->4)-L-guluronic acid, and polyM, i.e. poly-beta-D-mannuronic acid ?
4.2.2.11 poly(alpha-(1->4)-L-guluronic acid)
-
 Pseudoalteromonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 32000, SDS-PAGE Pseudoalteromonas sp.
4.2.2.11 ? x * 32000, SDS-PAGE Pseudoalteromonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Pseudoalteromonas sp.
4.2.2.3 Aly-SJ02
-
 Pseudoalteromonas sp.
4.2.2.3 polymannuronate lyase
-
 Pseudoalteromonas sp.
4.2.2.11 Aly-SJ02
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 50
-
-
 Pseudoalteromonas sp.
4.2.2.11 50
-
-
 Pseudoalteromonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 3 65 assay range Pseudoalteromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 half-life 41 min Pseudoalteromonas sp.
4.2.2.3 40
-
 half-life is 41 min Pseudoalteromonas sp.
4.2.2.3 50
-
 half-life 20 min Pseudoalteromonas sp.
4.2.2.3 50
-
 half-life is 20 min Pseudoalteromonas sp.
4.2.2.11 40
-
 half-life 41 min Pseudoalteromonas sp.
4.2.2.11 50
-
 half-life 20 min Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 8.5
-
-
 Pseudoalteromonas sp.
4.2.2.11 8.5
-
-
 Pseudoalteromonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5 10 assay range Pseudoalteromonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 4 11 at 25 °C for 20 min, the purified enzyme retains more than 50% activity at pH 7.0-10, aly-SJ02 is most stable at pH 8.0 Pseudoalteromonas sp.
4.2.2.3 8
-
 most stable at pH 8.0 Pseudoalteromonas sp.
4.2.2.11 8
-
 most stable at pH 8.0 Pseudoalteromonas sp.

General Information

EC Number General Information Comment Organism
4.2.2.3 evolution N-terminal sequence analysis suggest that aly-SJ02 may be an alginate lyase of polysaccharide lyase family 18 Pseudoalteromonas sp.