BRENDA - Enzyme Database

Phospholipase Dalpha from sunflower (Helianthus annuus): cloning and functional characterization

Moreno-Perez, A.J.; Martinez-Force, E.; Garces, R.; Salas, J.J.; J. Plant Physiol. 167, 503-511 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.4.4
gene HaPLDalpha, cloning from leaf DNA, DNA and amino acid sequence determination and analysis, expression of His-tagged PLDalpha in Escherichia coli
Helianthus annuus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
recombinant PLDalpha, dependent on
Helianthus annuus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Helianthus annuus
-
gene HaPLDalpha
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
recombinant His-tagged PLDalpha from Escherichia coli by nickel affinity chromatography to homogeneity
Helianthus annuus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
cotyledon
PLDalpha
Helianthus annuus
-
3.1.4.4
leaf
high expression level of PLDalpha
Helianthus annuus
-
3.1.4.4
root
PLDalpha
Helianthus annuus
-
3.1.4.4
seed
PLDalpha
Helianthus annuus
-
3.1.4.4
stem
high expression level of PLDalpha
Helianthus annuus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
716035
Helianthus annuus
choline + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
716035
Helianthus annuus
ethanolamine + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
716035
Helianthus annuus
glycerol + phosphatidate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6.5
-
recombinant PLDalpha
Helianthus annuus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
gene HaPLDalpha, cloning from leaf DNA, DNA and amino acid sequence determination and analysis, expression of His-tagged PLDalpha in Escherichia coli
Helianthus annuus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
recombinant PLDalpha, dependent on
Helianthus annuus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
recombinant His-tagged PLDalpha from Escherichia coli by nickel affinity chromatography to homogeneity
Helianthus annuus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.4
cotyledon
PLDalpha
Helianthus annuus
-
3.1.4.4
leaf
high expression level of PLDalpha
Helianthus annuus
-
3.1.4.4
root
PLDalpha
Helianthus annuus
-
3.1.4.4
seed
PLDalpha
Helianthus annuus
-
3.1.4.4
stem
high expression level of PLDalpha
Helianthus annuus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
716035
Helianthus annuus
choline + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylethanolamine + H2O
-
716035
Helianthus annuus
ethanolamine + phosphatidate
-
-
-
?
3.1.4.4
phosphatidylglycerol + H2O
-
716035
Helianthus annuus
glycerol + phosphatidate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
6.5
-
recombinant PLDalpha
Helianthus annuus
General Information
EC Number
General Information
Commentary
Organism
3.1.4.4
physiological function
D type phospholipases are enzymes that hydrolyze the head group of phospholipids to produce phosphatidic acid
Helianthus annuus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.4.4
physiological function
D type phospholipases are enzymes that hydrolyze the head group of phospholipids to produce phosphatidic acid
Helianthus annuus