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Literature summary extracted from

  • Smulevich, G.; Jakopitsch, C.; Droghetti, E.; Obinger, C.
    Probing the structure and bifunctionality of catalase-peroxidase (KatG) (2006), J. Inorg. Biochem., 100, 568-585.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.21 expressed in Escherichia coli Synechococcus sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.21
-
 Burkholderia pseudomallei
1.11.1.21
-
 Mycobacterium tuberculosis
1.11.1.21
-
 Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.21 D141A the mutant shows 1.5% of wild type catalase activity and 132% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 D141E the mutant shows 80% of wild type catalase activity and 143% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 D141N the mutant shows 10% of wild type catalase activity and 123% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 D152N the mutant shows 2.7% of wild type catalase activity and 234% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 D152S the mutant shows 5.7% of wild type catalase activity and 237% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 D152W the mutant shows 0.6% of wild type catalase activity and 672% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 D402E the mutant shows 0.6% of wild type catalase activity and 65% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 D402N the mutant shows 0.5% of wild type catalase activity and 56% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 E253D the mutant shows 42% of wild type catalase activity and 280% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 E253Q the mutant shows 25% of wild type catalase activity and 96% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 H106C the mutant shows 0.01% of wild type catalase activity and 1.7% of wild type peroxidase activity Escherichia coli
1.11.1.21 H106Y the mutant shows 0.008% of wild type catalase activity and 2.7% of wild type peroxidase activity Escherichia coli
1.11.1.21 H108Q the mutant shows 0.03% of wild type catalase activity and 47% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 H112A the mutant shows 0.02% of wild type catalase activity and 2.3% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 H112N the mutant shows 0.05% of wild type catalase activity and 2.3% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 H123E the mutant shows 0.03% of wild type catalase activity and 13% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 H123Q the mutant shows 0.02% of wild type catalase activity and 7.4% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 H257Y the mutant shows 0.05% of wild type catalase activity and 1.8% of wild type peroxidase activity Escherichia coli
1.11.1.21 H290Q the mutant shows 0.09% of wild type catalase activity and 5.6% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 I248F the mutant shows 12% of wild type catalase activity and 124% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 M264A the mutant shows 0.15% of wild type catalase activity and 160% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 M264L the mutant shows 0.02% of wild type catalase activity and 140% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 M275I the mutant shows 0.6% of wild type catalase activity and 640% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 N153A the mutant shows 6% of wild type catalase activity and 60% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 N153D the mutant shows 17% of wild type catalase activity and 130% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 N251L the mutant shows 33% of wild type catalase activity and 415% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 P252A the mutant shows 110% of wild type catalase activity and 97% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 R102C the mutant shows 0.5% of wild type catalase activity and 10% of wild type peroxidase activity Escherichia coli
1.11.1.21 R102K the mutant shows 1.8% of wild type catalase activity and 13% of wild type peroxidase activity Escherichia coli
1.11.1.21 R102L the mutant shows 1% of wild type catalase activity and 13% of wild type peroxidase activity Escherichia coli
1.11.1.21 R104L the mutant shows 0.06% of wild type catalase activity and 200% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 R108A the mutant shows 31% of wild type catalase activity and 23% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 R108K the mutant shows 8% of wild type catalase activity and 21% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 R119A the mutant shows 15% of wild type catalase activity and 12% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 R119N the mutant shows 0.5% of wild type catalase activity and 5% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 R418L the mutant shows 0.6% of wild type catalase activity and 192% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 R426A the mutant shows 4.3% of wild type catalase activity and 99% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 R426K the mutant shows 70% of wild type catalase activity and 97% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 R439A the mutant shows 4.9% of wild type catalase activity and 12% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 R439N the mutant shows 3.1% of wild type catalase activity and 100% wild type peroxidase activity Synechococcus sp.
1.11.1.21 S315T the mutant shows 52% of wild type catalase activity and 50% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 S324T the mutant shows 109% of wild type catalase activity and 94% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 S335T the mutant shows 99% of wild type catalase activity and 103% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 W105F the mutant shows 0.1% of wild type catalase activity and 288% of wild type peroxidase activity Escherichia coli
1.11.1.21 W105L the mutant shows 0.3% of wild type catalase activity and 197% of wild type peroxidase activity Escherichia coli
1.11.1.21 W111F the mutant shows 0.05% of wild type catalase activity and 75% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 W122A the mutant shows no catalase activity and 100% wild type peroxidase activity Synechococcus sp.
1.11.1.21 W122F the mutant shows no catalase activity and 90% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 W321F the mutant shows 38% of wild type catalase activity and 18% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 W341A the mutant shows 0.5% of wild type catalase activity and 25% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 W341F the mutant shows 42% of wild type catalase activity and 190% of wild type peroxidase activity Synechococcus sp.
1.11.1.21 Y229F the mutant shows 0.002% of wild type catalase activity and 1360% of wild type peroxidase activity Mycobacterium tuberculosis
1.11.1.21 Y238A the mutant shows 0.05% of wild type catalase activity and 140% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 Y238F the mutant shows 0.15% of wild type catalase activity and 64% of wild type peroxidase activity Burkholderia pseudomallei
1.11.1.21 Y249F the mutant shows 0.17% of wild type catalase activity and 121% of wild type peroxidase activity Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Burkholderia pseudomallei Q939D2
-
-
1.11.1.21 Escherichia coli
-
-
-
1.11.1.21 Mycobacterium tuberculosis P9WIE5
-
-
1.11.1.21 Mycobacterium tuberculosis H37Rv P9WIE5
-
-
1.11.1.21 Synechococcus sp. Q31MN3
-
-
1.11.1.21 Synechococcus sp. PCC 7942 Q31MN3
-
-

Synonyms

EC Number Synonyms Comment Organism
1.11.1.21 KatG
-
 Escherichia coli
1.11.1.21 KatG
-
 Burkholderia pseudomallei
1.11.1.21 KatG
-
 Mycobacterium tuberculosis
1.11.1.21 KatG
-
 Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 5.5
-
 peroxidase activity of KatG Escherichia coli
1.11.1.21 5.5
-
 peroxidase activity of KatG Burkholderia pseudomallei
1.11.1.21 5.5
-
 peroxidase activity of KatG Mycobacterium tuberculosis
1.11.1.21 5.5
-
 peroxidase activity of KatG Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Escherichia coli
1.11.1.21 heme
-
 Burkholderia pseudomallei
1.11.1.21 heme
-
 Mycobacterium tuberculosis
1.11.1.21 heme ferric and ferrous KatG show predominant five-coordinate high-spin heme. However, the ferric form shows also small amounts of both six-coordinate high-spin and six-coordinate low-spin, and the ferrous form also exhibits a 6-coordinate low-spin heme Synechococcus sp.