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Literature summary extracted from

  • Nagahama, K.; Ogawa, T.; Fujii, T.; Tazaki, M.; Tanase, S.; Morino, Y.; Fukuda, H.
    Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. Phaseolicola PK2 (1991), J. Gen. Microbiol., 137, 2281-2286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.19 D-arginine 3% of the activity with L-arginine Pseudomonas syringae
1.13.12.19 L-arginine highly specific for cofactor L-arginine, KM value 0.018 mM Pseudomonas syringae
1.13.12.19 L-canavanine sulfate 7% of the activity with L-arginine Pseudomonas syringae
1.14.20.7 D-arginine 3% of the activity with L-arginine Pseudomonas syringae
1.14.20.7 L-arginine highly specific for cofactor L-arginine, KM value 0.018 mM Pseudomonas syringae
1.14.20.7 L-canavanine sulfate 7% of the activity with L-arginine Pseudomonas syringae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.19 4,5-dihydroxy-1,3-benzene disulfonic acid 1 mM, 0.8% residual activity Pseudomonas syringae
1.13.12.19 5,5'-dithio-bis(2-nitrobenzoate) 1 mM, 0.7% residual activity Pseudomonas syringae
1.13.12.19 CoCl2 1 mM, 20% residual activity Pseudomonas syringae
1.13.12.19 CuSO4 1 mM, 50% residual activity Pseudomonas syringae
1.13.12.19 EDTA 1 mM, 1% residual activity Pseudomonas syringae
1.13.12.19 H2O2 1 mM, 0.7% residual activity Pseudomonas syringae
1.13.12.19 MnCl2 1 mM, 6% residual activity Pseudomonas syringae
1.13.12.19 n-propyl gallate 1 mM, 1% residual activity Pseudomonas syringae
1.13.12.19 Sodium azide 1 mM, 90% residual activity Pseudomonas syringae
1.14.20.7 4,5-dihydroxy-1,3-benzenedisulfonic acid 1 mM, 0.8% residual activity Pseudomonas syringae
1.14.20.7 5,5'-dithio-bis(2-nitrobenzoate) 1 mM, 0.7% residual activity Pseudomonas syringae
1.14.20.7 CoCl2 1 mM, 20% residual activity Pseudomonas syringae
1.14.20.7 CuSO4 1 mM, 50% residual activity Pseudomonas syringae
1.14.20.7 EDTA 1 mM, 1% residual activity Pseudomonas syringae
1.14.20.7 H2O2 1 mM, 0.7% residual activity Pseudomonas syringae
1.14.20.7 MnCl2 1 mM, 6% residual activity Pseudomonas syringae
1.14.20.7 n-propyl gallate 1 mM, 1% residual activity Pseudomonas syringae
1.14.20.7 Sodium azide 1 mM, 90% residual activity Pseudomonas syringae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.19 0.019
-
 2-oxoglutarate pH 8.0, 25°C Pseudomonas syringae
1.14.20.7 0.019
-
 2-oxoglutarate pH 8.0, 25°C Pseudomonas syringae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.19 Fe2+ required, KM value 0.059 mM Pseudomonas syringae
1.14.20.7 Fe2+ required, KM value 0.059 mM Pseudomonas syringae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.19 36000
-
 gel fitlration Pseudomonas syringae
1.13.12.19 42000
-
 1 * 42000, SDS-PAGE Pseudomonas syringae
1.14.20.7 36000
-
 gel filtration Pseudomonas syringae
1.14.20.7 42000
-
 1 * 42000, SDS-PAGE Pseudomonas syringae

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.19 Pseudomonas syringae P32021 pv. phaseolicola PK2, enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, reactions of EC 1.13.12.19 and EC 1.14.11.34
-
1.14.20.7 Pseudomonas syringae P32021 enzyme catalyzes the formation of ethylene and succinate from 2-oxoglutarate, reactions of EC 1.13.12.19 and EC 1.14.11.34; pv. phaseolicola PK2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.19 from cell-free extract Pseudomonas syringae
1.14.20.7 from cell-free extract Pseudomonas syringae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.19 660
-
 pH 8.0, 25°C Pseudomonas syringae
1.14.20.7 660
-
 pH 8.0, 25°C Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.19 2-oxoglutarate + O2 enzyme is highly specific for substrate 2-oxoglutarate Pseudomonas syringae ethylene + 3 CO2 + H2O
-
 ?
1.13.12.19 additional information presence of 2-oxoglutarate, L-arginine, Fe2+ and oxygen is essential for the enzymic reaction Pseudomonas syringae ?
-
 ?
1.14.20.7 2-oxoglutarate + L-arginine + O2 overall reaction, enzyme is highly specific for substrate 2-oxoglutarate. Presence of 2-oxoglutarate, L-arginine, Fe2+ and oxygen is essential for the enzymic reaction Pseudomonas syringae succinate + CO2 + guanidine + (S)-1-pyrroline-5-carboxylate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.19 monomer 1 * 42000, SDS-PAGE Pseudomonas syringae
1.14.20.7 monomer 1 * 42000, SDS-PAGE Pseudomonas syringae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.19 20 25
-
 Pseudomonas syringae
1.14.20.7 20 25
-
 Pseudomonas syringae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.19 30
-
 stable below Pseudomonas syringae
1.14.20.7 30
-
 stable below Pseudomonas syringae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.19 7 7.5
-
 Pseudomonas syringae
1.14.20.7 7 7.5
-
 Pseudomonas syringae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.12.19 Pseudomonas syringae isoelectric focusing
-
 5.9
1.14.20.7 Pseudomonas syringae isoelectric focusing
-
 5.9