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Literature summary extracted from

  • Allardyce, B.J.; Linton, S.M.; Saborowski, R.
    The last piece in the cellulase puzzle: the characterisation of beta-glucosidase from the herbivorous gecarcinid land crab Gecarcoidea natalis (2010), J. Exp. Biol., 213, 2950-2957.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.74 130300
-
gel filtration Gecarcoidea natalis
3.2.1.74 169300
-
calculated from amino acid sequence Gecarcoidea natalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Gecarcoidea natalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration Gecarcoidea natalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.74 midgut gland
-
Gecarcoidea natalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.74 0.067
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication Gecarcoidea natalis
3.2.1.74 0.228
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication Gecarcoidea natalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 cellobiose + H2O glucosyl transfer reaction Gecarcoidea natalis D-glucose + cellotetraose + cellotriose
-
?
3.2.1.74 cellotetraose + H2O highest substrate preference for cellotriose and cellotetraose Gecarcoidea natalis D-glucose + cellotriose + cellobiose
-
?
3.2.1.74 cellotriose + H2O highest substrate preference for cellotriose and cellotetraose Gecarcoidea natalis D-glucose + cellobiose
-
?
3.2.1.74 cellulose + H2O the glucohydrolase attaches directly to crystalline cellulose to release D-glucose from free chain ends Gecarcoidea natalis D-glucose + ?
-
?
3.2.1.74 additional information no activity is detected towards carboxymethyl cellulose Gecarcoidea natalis ?
-
?
3.2.1.74 additional information the enzyme is able to synthesise some cellotetraose by the transglycosylation of smaller substrates Gecarcoidea natalis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 1,4-beta-D-glucan glucanohydrolase
-
Gecarcoidea natalis
3.2.1.74 glucohydrolase
-
Gecarcoidea natalis