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Literature summary extracted from

  • Amann, P.M.; Luo, C.; Owen, R.W.; Hofmann, C.; Freudenberger, M.; Schadendorf, D.; Eichmueller, S.B.; Bazhin, A.V.
    Vitamin A metabolism in benign and malignant melanocytic skin cells: importance of lecithin/retinol acyltransferase and RPE65 (2012), J. Cell. Physiol., 227, 718-728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 LRAT, quantitative real-time RT-PCR expression analysis in melanoma cell lines Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.135 additional information siRNA knockdown of LRAT and RPE65 expression Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein] Homo sapiens
-
 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Homo sapiens
-
-
-
3.1.1.64 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.135 HaCaT cell
-
 Homo sapiens
-
2.3.1.135 keratinocyte
-
 Homo sapiens
-
2.3.1.135 Ma-Mel-04 cell
-
 Homo sapiens
-
2.3.1.135 Ma-Mel-11 cell
-
 Homo sapiens
-
2.3.1.135 Ma-Mel-12 cell
-
 Homo sapiens
-
2.3.1.135 melanocyte epidermal Homo sapiens
-
2.3.1.135 melanoma cell diverse cell lines Homo sapiens
-
2.3.1.135 MeWo cell
-
 Homo sapiens
-
2.3.1.135 retinoblastoma cell
-
 Homo sapiens
-
2.3.1.135 SK-MEL-23 cell
-
 Homo sapiens
-
2.3.1.135 skin
-
 Homo sapiens
-
2.3.1.135 UKRV-Mel-27 cell
-
 Homo sapiens
-
2.3.1.135 Y-79 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-01 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-04 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-05 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-11 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-12 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-17 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-19 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-37b cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-42b cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-47 cell
-
 Homo sapiens
-
3.1.1.64 Ma-Mel-52 cell
-
 Homo sapiens
-
3.1.1.64 melanoma cell malignant melanoma cells are able to esterify all-trans retinol and subsequently isomerize all-trans retinyl esters into 11-cis retinol, whereas melanocytes are not able to catalyze these reactions Homo sapiens
-
3.1.1.64 MeWo cell
-
 Homo sapiens
-
3.1.1.64 additional information melanocytes do not express this enzyme at the protein level Homo sapiens
-
3.1.1.64 SK-MEL-23 cell
-
 Homo sapiens
-
3.1.1.64 UKRV-Mel-06a cell
-
 Homo sapiens
-
3.1.1.64 UKRV-Mel-2 cell
-
 Homo sapiens
-
3.1.1.64 UKRV-Mel-21a cell
-
 Homo sapiens
-
3.1.1.64 UKRV-Mel-27 cell
-
 Homo sapiens
-
3.1.1.64 UKRV-Mel-31 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein]
-
 Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
 ?
2.3.1.135 phosphatidylcholine + retinol-[cellular-retinol-binding-protein] esterification of all-trans-retinol Homo sapiens 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.135 lecithin/retinol acyltransferase
-
 Homo sapiens
2.3.1.135 LRAT
-
 Homo sapiens
3.1.1.64 Rpe65
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.3.1.135 Homo sapiens expression of LRAT and RPE65 can be modulated by retinoids additional information

General Information

EC Number General Information Comment Organism
2.3.1.135 metabolism the key step of vitamin A metabolism is the esterification of all-trans retinol, catalyzed by lecithin/retinol acyltransferase, LRAT. Vitamin A metabolism in benign and malignant melanocytic skin cells with regard to expression, functional activity of LRAT, RPE65, and cRBP2 and their regulation, overview Homo sapiens
2.3.1.135 additional information malignant melanoma cells are able to esterify all-trans retinol and subsequently isomerize all-trans retinyl esters into 11-cis retinol, whereas their benign counterpart melanocytes are not able to catalyze these reactions Homo sapiens
2.3.1.135 physiological function activities of LRAT and RPE65 may be important for removal of all-trans retinal which is the substrate for retinoic acid production in skin cells. Decreasing cellular amount of retinoic acid and its precursor molecules might result in a change of gene regulation Homo sapiens
3.1.1.64 physiological function RPE65 is important for vitamin A metabolism in melanoma cells and in keratinocytes. Activity of RPE65 is important for removal of all-trans retinal which is the substrate for retinoic acid production in skin cells Homo sapiens