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Literature summary extracted from

  • Zehentgruber, D.; Dragan, C.A.; Bureik, M.; Luetz, S.
    Challenges of steroid biotransformation with human cytochrome P450 monooxygenase CYP21 using resting cells of recombinant Schizosaccharomyces pombe (2010), J. Biotechnol., 146, 179-185.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.16 biotechnology the CYP21 expression model system using resting Schizosaccharomyces pombe cells can be used for biotransformations Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.16 gene CYP21, expression in Schizosaccharomyces pombe strain CAD18, method optimization and evaluation, and quantification of the intracellular redox cofactor pool in transformed cells, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.16 17alpha-hydroxyprogesterone inhibits above 0.2mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.16 additional information
-
 additional information reaction kinetic, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.16 Fe2+ a cytochrome P450 monooxygenase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.16 17-hydroxyprogesterone + NADPH + H+ + O2 Homo sapiens
-
 11-deoxycortisol + NADP+ + H2O
-
 ?
1.14.14.16 additional information Homo sapiens CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.16 Homo sapiens P08686
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.16 17-hydroxyprogesterone + NADPH + H+ + O2
-
 Homo sapiens 11-deoxycortisol + NADP+ + H2O
-
 ?
1.14.14.16 17-hydroxyprogesterone + NADPH + H+ + O2 the redox cofactor provides the electrons required for a P450-dependent hydroxylation. Maximal CYP 21 activity is observed in presence of low substrate concentrations of 0.2 mM. Higher substrate concentrations reduce the activity and inhibit the enzyme Homo sapiens 11-deoxycortisol + NADP+ + H2O
-
 ?
1.14.14.16 additional information CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe Homo sapiens ?
-
 ?
1.14.14.16 additional information reaction method optimization, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.16 CYP21
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.16 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.16 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.16 cytochrome P450 Cyp21 is a cytochrome P450 monooxygenase Homo sapiens
1.14.14.16 NADPH the redox cofactor provides the electrons required for a P450-dependent hydroxylation Homo sapiens