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Literature summary extracted from

  • Shapiro, A.B.; Eakin, A.E.; Walkup, G.K.; Rivin, O.
    A high-throughput fluorescence resonance energy transfer-based assay for DNA ligase (2011), J. Biomol. Screen., 16, 486-493.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.5.1.2 (NH4)2SO4 with 30 mM (NH4)2SO4, the activity is maximal Haemophilus influenzae
6.5.1.2 KCl activation by KCl is observed at (NH4)2SO4 concentrations below 30 mM, in the absence of (NH4)2SO4 ligase activity is activated by KCl over 0-100 mM, and at 100 mM KCl, further activation occurs at up to 12 mM (NH4)2SO4 Haemophilus influenzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2
-
 Haemophilus influenzae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.2 (NH4)2SO4 (NH4)2SO4 concentrations higher than 30 mM are inhibitory. At 100 mM KCl, concentrations of (NH4)2SO4 above 12 mM are inhibitory Haemophilus influenzae
6.5.1.2 1,N6-etheno-NAD+
-
 Haemophilus influenzae
6.5.1.2 KCl inhibition by KCl occurs at greater than 30 mM (NH4)2SO Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Mg2+ required for activity, maximal activity occurs with 10-20 mM MgCl2 in the presence of 30 mM each of KCl and (NH4)2SO4 Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Haemophilus influenzae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.2 NAD+-dependent DNA ligase
-
 Haemophilus influenzae

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+
-
 Haemophilus influenzae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.5.1.2 0.054
-
 pH and temperature not specified in the publication Haemophilus influenzae 1,N6-etheno-NAD+