BRENDA - Enzyme Database show

An interaction between L-prostaglandin D synthase and arrestin increases PGD2 production

Mathurin, K.; Gallant, M.A.; Germain, P.; Allard-Chamard, H.; Brisson, J.; Iorio-Morin, C.; de Brum Fernandes, A.; Caron, M.G.; Laporte, S.A.; Parent, J.L.; J. Biol. Chem. 286, 2696-2706 (2011)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
arrestin-3
arrestin-3 promotes prostaglandin D2 production by L-PGDS in vitro. Incubation of L-PGDS with arrestin-3 amino acids 56-100 enhances prostaglandin D2 production by 145%
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli C41(DE3) cells
Homo sapiens
5.3.99.2
expressed in Escherichia coli C41(DE3) cells
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
SeCl4
-
Homo sapiens
5.3.99.2
SeCl4
-
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.2
cytoplasm
-
Homo sapiens
5737
-
5.3.99.2
cytoplasm
-
Mus musculus
5737
-
5.3.99.2
nucleus
perinuclear L-type prostaglandin synthase/arrestin-3 co-localization is observed in prostaglandin D2-producing MG-63 cells
Homo sapiens
5634
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
25000
-
SDS-PAGE
Homo sapiens
5.3.99.2
25000
-
SDS-PAGE
Mus musculus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Homo sapiens
-
-
-
5.3.99.2
Mus musculus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
Ni-NTA agarose column chromatography and glutathione-Sepharose column chromatography
Homo sapiens
5.3.99.2
Ni-NTA agarose column chromatography and glutathione-Sepharose column chromatography
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.2
MEF cell
-
Mus musculus
-
5.3.99.2
MG-63 cell
-
Homo sapiens
-
5.3.99.2
osteoblast
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
715626
Mus musculus
Prostaglandin D2
-
-
-
?
5.3.99.2
Prostaglandin H2
-
715626
Homo sapiens
Prostaglandin D2
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
additional information
L-PGDS is glutathione-independent
Homo sapiens
5.3.99.2
additional information
L-PGDS is glutathione-independent
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
arrestin-3
arrestin-3 promotes prostaglandin D2 production by L-PGDS in vitro. Incubation of L-PGDS with arrestin-3 amino acids 56-100 enhances prostaglandin D2 production by 145%
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli C41(DE3) cells
Homo sapiens
5.3.99.2
expressed in Escherichia coli C41(DE3) cells
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.2
additional information
L-PGDS is glutathione-independent
Homo sapiens
5.3.99.2
additional information
L-PGDS is glutathione-independent
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.2
SeCl4
-
Homo sapiens
5.3.99.2
SeCl4
-
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.2
cytoplasm
-
Homo sapiens
5737
-
5.3.99.2
cytoplasm
-
Mus musculus
5737
-
5.3.99.2
nucleus
perinuclear L-type prostaglandin synthase/arrestin-3 co-localization is observed in prostaglandin D2-producing MG-63 cells
Homo sapiens
5634
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.2
25000
-
SDS-PAGE
Homo sapiens
5.3.99.2
25000
-
SDS-PAGE
Mus musculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
Ni-NTA agarose column chromatography and glutathione-Sepharose column chromatography
Homo sapiens
5.3.99.2
Ni-NTA agarose column chromatography and glutathione-Sepharose column chromatography
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.2
MEF cell
-
Mus musculus
-
5.3.99.2
MG-63 cell
-
Homo sapiens
-
5.3.99.2
osteoblast
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
715626
Mus musculus
Prostaglandin D2
-
-
-
?
5.3.99.2
Prostaglandin H2
-
715626
Homo sapiens
Prostaglandin D2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
5.3.99.2
malfunction
co-localization of arrestin-3 with L-PGDS is drastically reduced in MG-63 cells when L-PGDS activity is inhibited
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
5.3.99.2
malfunction
co-localization of arrestin-3 with L-PGDS is drastically reduced in MG-63 cells when L-PGDS activity is inhibited
Homo sapiens