EC Number | Application | Comment | Organism |
---|---|---|---|
6.3.3.2 | analysis | development of a functional complementation assay for 5-CHO-THF metabolism in Escherichia coli, based on deleting the gene encoding 5-FCL. The deletion mutant accumulates 5-formyltetrahydrofolate and,with glycine as sole nitrogen source, shows a growth defect, both phenotypes are complemented by bacterial or archaeal genes encoding glutamate formiminotransferase. Glutamate formiminotransferases functionally replace 5-formyltetrahydrofolate cyclo-ligases in certain prokaryotes | Escherichia coli |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.5 | glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-CHO-THF cycloligase deletion mutant with strain MG1655 background | Streptococcus pyogenes |
2.1.2.5 | glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background | Thermoplasma acidophilum |
2.1.2.5 | glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background | Picrophilus torridus |
2.1.2.5 | glutamate formiminotransferase, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, functional complementation of an Escherichia coli DELTAygfA 5-N-formimidoyltetrahydrofolate cycloligase deletion mutant with strain MG1655 background | Candidatus Koribacter versatilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.5 | 0.0004 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Picrophilus torridus | |
2.1.2.5 | 0.0007 | - |
N-formimidoyl-L-Glu-L-Glu-L-Glu | pH 7.3, 30°C | Picrophilus torridus | |
2.1.2.5 | 0.0018 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Candidatus Koribacter versatilis | |
2.1.2.5 | 0.0037 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Thermoplasma acidophilum | |
2.1.2.5 | 0.0038 | - |
N-formimidoyl-L-Glu-L-Glu-L-Glu | pH 7.3, 30°C | Thermoplasma acidophilum | |
2.1.2.5 | 0.0048 | - |
N-formimidoyl-L-Glu-L-Glu-L-Glu | pH 7.3, 30°C | Streptococcus pyogenes | |
2.1.2.5 | 0.0054 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Streptococcus pyogenes | |
2.1.2.5 | 0.0058 | - |
N-formimidoyl-L-Glu-L-Glu-L-Glu | pH 7.3, 30°C | Candidatus Koribacter versatilis | |
2.1.2.5 | 0.034 | - |
L-glutamate | pH 7.3, 30°C | Picrophilus torridus | |
2.1.2.5 | 0.113 | - |
L-glutamate | pH 7.3, 30°C | Thermoplasma acidophilum | |
2.1.2.5 | 0.206 | - |
L-glutamate | pH 7.3, 30°C | Candidatus Koribacter versatilis | |
2.1.2.5 | 1.03 | - |
L-glutamate | pH 7.3, 30°C | Streptococcus pyogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Thermoplasma acidophilum | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Streptococcus pyogenes | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Picrophilus torridus | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Candidatus Koribacter versatilis | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Candidatus Koribacter versatilis Ellin345 | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | Streptococcus pyogenes M1 | - |
tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.5 | Candidatus Koribacter versatilis | - |
- |
- |
2.1.2.5 | Candidatus Koribacter versatilis Ellin345 | - |
- |
- |
2.1.2.5 | no activity in Herpetosiphon aurantiacus | - |
the gene encoding glycine formimidoyltransferase is an inactive pseudogene, the histidine utilization hut operon in Herpetosiphon aurantiacus is entirely broken up, suggesting that this pathway no longer functions | - |
2.1.2.5 | Picrophilus torridus | - |
- |
- |
2.1.2.5 | Streptococcus pyogenes | - |
- |
- |
2.1.2.5 | Streptococcus pyogenes M1 | - |
- |
- |
2.1.2.5 | Thermoplasma acidophilum | - |
- |
- |
6.3.3.2 | Escherichia coli | A0A061KWR9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.5 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography | Thermoplasma acidophilum |
2.1.2.5 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography | Streptococcus pyogenes |
2.1.2.5 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography | Picrophilus torridus |
2.1.2.5 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by affinity chormatography | Candidatus Koribacter versatilis |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.2.5 | -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, loss of 80% activity after 8 weeks | Candidatus Koribacter versatilis |
2.1.2.5 | -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks | Thermoplasma acidophilum |
2.1.2.5 | -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks | Streptococcus pyogenes |
2.1.2.5 | -80°C, purified desalted recombinant enzyme, 100 mM K-phosphate, pH 7.3, 10% v/v glycerol, flash frozen in liquid N2, stable for at least 8 weeks | Picrophilus torridus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Thermoplasma acidophilum | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Streptococcus pyogenes | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Picrophilus torridus | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Candidatus Koribacter versatilis | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Candidatus Koribacter versatilis Ellin345 | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | 5-formimidoyltetrahydrofolate + L-glutamate | - |
Streptococcus pyogenes M1 | tetrahydrofolate + N-formimidoyl-L-glutamate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Thermoplasma acidophilum | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Streptococcus pyogenes | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Picrophilus torridus | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Candidatus Koribacter versatilis | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Candidatus Koribacter versatilis Ellin345 | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? | |
2.1.2.5 | N-formimidoyl-L-Glu-L-Glu-L-Glu + tetrahydrofolate | - |
Streptococcus pyogenes M1 | N-formimidoyl-L-Glu-L-Glu + 5-formimidoyltetrahydrofolate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.2.5 | glutamate formiminotransferase | - |
Thermoplasma acidophilum |
2.1.2.5 | glutamate formiminotransferase | - |
Streptococcus pyogenes |
2.1.2.5 | glutamate formiminotransferase | - |
Picrophilus torridus |
2.1.2.5 | glutamate formiminotransferase | - |
Candidatus Koribacter versatilis |
6.3.3.2 | ygfA | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.5 | 30 | - |
assay at | Thermoplasma acidophilum |
2.1.2.5 | 30 | - |
assay at | Streptococcus pyogenes |
2.1.2.5 | 30 | - |
assay at | Picrophilus torridus |
2.1.2.5 | 30 | - |
assay at | Candidatus Koribacter versatilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.5 | 0.0022 | - |
L-glutamate | pH 7.3, 30°C | Streptococcus pyogenes | |
2.1.2.5 | 0.0022 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Streptococcus pyogenes | |
2.1.2.5 | 0.0045 | - |
L-glutamate | pH 7.3, 30°C | Thermoplasma acidophilum | |
2.1.2.5 | 0.0045 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Thermoplasma acidophilum | |
2.1.2.5 | 0.0092 | - |
L-glutamate | pH 7.3, 30°C | Candidatus Koribacter versatilis | |
2.1.2.5 | 0.0092 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Candidatus Koribacter versatilis | |
2.1.2.5 | 0.0193 | - |
L-glutamate | pH 7.3, 30°C | Picrophilus torridus | |
2.1.2.5 | 0.0193 | - |
5-formimidoyltetrahydrofolate | pH 7.3, 30°C | Picrophilus torridus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.5 | 7.3 | - |
assay at | Thermoplasma acidophilum |
2.1.2.5 | 7.3 | - |
assay at | Streptococcus pyogenes |
2.1.2.5 | 7.3 | - |
assay at | Picrophilus torridus |
2.1.2.5 | 7.3 | - |
assay at | Candidatus Koribacter versatilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.2.5 | metabolism | glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview | Thermoplasma acidophilum |
2.1.2.5 | metabolism | glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview | Streptococcus pyogenes |
2.1.2.5 | metabolism | glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview | Picrophilus torridus |
2.1.2.5 | metabolism | glutamate formiminotransferase is a histidine breakdown enzyme. 5-Formimidoyltetrahydrofolate in C1-metabolism, overview | Candidatus Koribacter versatilis |
2.1.2.5 | physiological function | utilization of supplied 5-formyltetrahydrofolate by Streptococcus pyogenes requires expression of the native glutamate formiminotransferase | Streptococcus pyogenes |