BRENDA - Enzyme Database show

Identification of a novel palmitylation site essential for membrane association and isomerohydrolase activity of RPE65

Takahashi, Y.; Moiseyev, G.; Ablonczy, Z.; Chen, Y.; Crouch, R.K.; Ma, J.X.; J. Biol. Chem. 284, 3211-3218 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.64
additional information
the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65
Bos taurus
3.1.1.64
additional information
the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
C106A
the mutation does not affect the palmitylation status of the fragment 98-118, the mutant remains associated with the membrane. The mutant has an intact enzymatic activity similar to that of wild type enzyme
Homo sapiens
3.1.1.64
C112A
the mutation abolishes palmitylation in fragment 98-118 and dissociates RPE65 from the membrane. The mutant has no enzymatic activity
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
-
Homo sapiens
16020
-
3.1.1.64
membrane
-
Bos taurus
16020
-
3.1.1.64
microsome
-
Bos taurus
-
-
3.1.1.64
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
61000
-
x * 61000, estimated from amino acid sequence
Bos taurus
3.1.1.64
61000
-
x * 61000, estimated from amino acid sequence
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Bos taurus
-
-
-
3.1.1.64
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Bos taurus
-
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715501
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
-
715501
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 61000, estimated from amino acid sequence
Bos taurus
3.1.1.64
?
x * 61000, estimated from amino acid sequence
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.64
additional information
the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65
Bos taurus
3.1.1.64
additional information
the Cys-112 palmitylation site is essential for the membrane association and activity of RPE65
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.1.64
C106A
the mutation does not affect the palmitylation status of the fragment 98-118, the mutant remains associated with the membrane. The mutant has an intact enzymatic activity similar to that of wild type enzyme
Homo sapiens
3.1.1.64
C112A
the mutation abolishes palmitylation in fragment 98-118 and dissociates RPE65 from the membrane. The mutant has no enzymatic activity
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
membrane
-
Homo sapiens
16020
-
3.1.1.64
membrane
-
Bos taurus
16020
-
3.1.1.64
microsome
-
Bos taurus
-
-
3.1.1.64
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.64
61000
-
x * 61000, estimated from amino acid sequence
Bos taurus
3.1.1.64
61000
-
x * 61000, estimated from amino acid sequence
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Bos taurus
-
3.1.1.64
retinal pigment epithelium
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715501
Homo sapiens
11-cis-retinol + a fatty acid
-
-
-
?
3.1.1.64
all-trans-retinyl ester + H2O
-
715501
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.64
?
x * 61000, estimated from amino acid sequence
Bos taurus
3.1.1.64
?
x * 61000, estimated from amino acid sequence
Homo sapiens