BRENDA - Enzyme Database show

RPE65 is an iron(II)-dependent isomerohydrolase in the retinoid visual cycle

Moiseyev, G.; Takahashi, Y.; Chen, Y.; Gentleman, S.; Redmond, T.M.; Crouch, R.K.; Ma, J.X.; J. Biol. Chem. 281, 2835-2840 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
1,10-phenanthroline
dose-dependent inhibition
Bos taurus
3.1.1.64
2,2'-bipyridine
dose-dependent inhibition
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
Fe2+ is essential for the isomerohydrolase activity
Bos taurus
3.1.1.64
additional information
not affected by Fe(III) citrate, Fe3+, Cu2+, Zn2+, or Mg2+
Bos taurus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.64
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.64
DEAE-Sepharose column chromatography
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715484
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.1.64
0.2
-
in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37C
Bos taurus
1,10-phenanthroline
3.1.1.64
0.5
-
in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37C
Bos taurus
2,2'-bipyridine
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
expressed in 293A-LRAT cells
Bos taurus
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.1.64
0.2
-
in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37C
Bos taurus
1,10-phenanthroline
3.1.1.64
0.5
-
in 10 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane, pH 8.0, 100 mM NaCl, at 37C
Bos taurus
2,2'-bipyridine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.64
1,10-phenanthroline
dose-dependent inhibition
Bos taurus
3.1.1.64
2,2'-bipyridine
dose-dependent inhibition
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.64
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.64
Fe2+
Fe2+ is essential for the isomerohydrolase activity
Bos taurus
3.1.1.64
additional information
not affected by Fe(III) citrate, Fe3+, Cu2+, Zn2+, or Mg2+
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.64
DEAE-Sepharose column chromatography
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.64
retinal pigment epithelium
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.64
all-trans-retinyl ester + H2O
-
715484
Bos taurus
11-cis-retinol + a fatty acid
-
-
-
?