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Literature summary extracted from

  • Raschle, T.; Amrhein, N.; Fitzpatrick, T.B.
    On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis (2005), J. Biol. Chem., 280, 32291-32300.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6
-
 Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.6 acivicin inhibition of the glutaminase domain YuaaD, and thus pyridoxal 5'-phosphate synthesis Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.6 0.068
-
 D-ribose 5-phosphate pH 8.0, 37°C Bacillus subtilis
4.3.3.6 0.077
-
 D-glyceraldehyde 3-phosphate pH 8.0, 37°C Bacillus subtilis
4.3.3.6 0.99
-
 L-glutamine pH 8.0, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.3.6 additional information 1.0 mM Mn2+, Mg2+, Co2+, Ca2+, Ni2+, Fe3+, Fe2+, Cu3+, Cu2+, or Zn2+ has no effect on activity Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine Bacillus subtilis the enzyme is involved in vitamin B6 biosynthesis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine the enzyme is involved in vitamin B6 biosynthesis Bacillus subtilis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine two proteins form a complex that functions as a glutamine amidotransferase, with YaaE as the glutaminase domain and YaaD as the acceptor and pyridoxal 5'-phosphate synthesis domain. The synthase reaction can also utilize an external ammonium source but, in contrast to other glutamine amidotransferases, is dependent on YaaE under certain conditions Bacillus subtilis pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 two proteins form a complex that functions as a glutamine amidotransferase, with YaaE as the glutaminase domain and YaaD as the acceptor and pyridoxal 5'-phosphate synthesis domain. Both the glutaminase and synthase reactions are dependent on the respective protein partner. The synthase reaction can also utilize an external ammonium source but, in contrast to other glutamine amidotransferases, is dependent on YaaE under certain conditions Bacillus subtilis pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?
4.3.3.6 L-glutamine + H2O two proteins form a complex that functions as a glutamine amidotransferase, with YaaE as the glutaminase domain and YaaD as the acceptor and pyridoxal 5'-phosphate synthesis domain. Glutaminase activity of YaaE is only detected in the presence of its partner protein YaaD. A 1:1 stoichiometry of both proteins appears to be optimal for activity Bacillus subtilis L-glutamate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.6 37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.3.6 0.00033
-
 D-ribose 5-phosphate pH 8.0, 37°C Bacillus subtilis
4.3.3.6 0.127
-
 L-glutamine pH 8.0, 37°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.6 8
-
 assay at Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.3.3.6 0.044
-
 D-ribose 5-phosphate pH 8.0, 37°C Bacillus subtilis
4.3.3.6 0.128
-
 L-glutamine pH 8.0, 37°C Bacillus subtilis