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Literature summary extracted from

  • Iwai, T.; Kuramitsu, S.; Masui, R.
    The Nudix hydrolase Ndx1 from Thermus thermophilus HB8 is a diadenosine hexaphosphate hydrolase with a novel activity (2004), J. Biol. Chem., 279, 21732-21739.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.61 overexpressed in Escherichia coli Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.61 E46Q 2200fold reduction in kcat Thermus thermophilus
3.6.1.61 E49Q mutation has very little effect on activity Thermus thermophilus
3.6.1.61 E50Q 130000fold reduction in kcat Thermus thermophilus
3.6.1.61 W26S no decrease in fluorescence intensity upon the addition of ATP Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.61 ATP
-
 Thermus thermophilus
3.6.1.61 dATP
-
 Thermus thermophilus
3.6.1.61 fluoride non-competitive inhibition Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.61 0.0014
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.0, 25°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.61 Co2+ Ndx1 activity requires the presence of the divalent cations Mn2+, Mg2+, Zn2+, and Co2+ Thermus thermophilus
3.6.1.61 Mg2+ Ndx1 activity requires the presence of the divalent cations Mn2+, Mg2+, Zn2+, and Co2+ Thermus thermophilus
3.6.1.61 Mn2+ Ndx1 activity requires the presence of the divalent cations Mn2+, Mg2+, Zn2+, and Co2+ Thermus thermophilus
3.6.1.61 additional information Ca2+, Ni2+, and Cu2+ are not able to activate Ndx1 Thermus thermophilus
3.6.1.61 Zn2+ Ndx1 activity requires the presence of the divalent cations Mn2+, Mg2+, Zn2+, and Co2+ Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.61 14170
-
 1 * 14170, calculated from sequence Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.61 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O Thermus thermophilus
-
 2 ATP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.61 Thermus thermophilus Q75UV1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.61
-
 Thermus thermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.6.1.61 P1,P4-bis(5'-adenosyl)tetraphosphate + H2O = ATP + AMP nucleophilic double, not single, displacement catalytic forward reaction mechanism via oxocarbenium ion intermediate, detailed overview Thermus thermophilus
a
3.6.1.61 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = ATP + ADP (2) Thermus thermophilus
a
3.6.1.61 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = 2 ATP (1) Thermus thermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.61 P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
 Thermus thermophilus ATP + AMP
-
 ?
3.6.1.61 P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
-
 Thermus thermophilus ATP + ADP
-
 ?
3.6.1.61 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O
-
 Thermus thermophilus 2 ATP
-
 ?
3.6.1.61 P1,P6-bis(5'-adenosyl)hexaphosphate + H2O P1,P6-bis(5'-adenosyl)hexaphosphate is the most preferred substrate. Glu46 and Glu50 are conserved residues in the Nudix motif and are involved in catalysis Thermus thermophilus 2 ATP
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.61 monomer 1 * 14170, calculated from sequence Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.61 Ndx1
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.61 70
-
 hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.61 95
-
 pH 7.5, stable up to Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.61 4.1
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.0, 25°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.61 8
-
 hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate Thermus thermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.6.1.61 2 12 25°C, stable Thermus thermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.61 0.013
-
 ATP pH 7.0, 25°C Thermus thermophilus
3.6.1.61 0.041
-
 dATP pH 7.0, 25°C Thermus thermophilus
3.6.1.61 0.424
-
 fluoride pH 7.0, 25°C Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.61 Thermus thermophilus calculated from sequence
-
 4.8

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.61 0.08
-
 pH 7.0, 25°C Thermus thermophilus fluoride

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.61 2929
-
 P1,P6-bis(5'-adenosyl)hexaphosphate pH 7.0, 25°C Thermus thermophilus