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Literature summary extracted from

  • Kasai, D.; Masai, E.; Miyauchi, K.; Katayama, Y.; Fukuda, M.
    Characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by Sphingomonas paucimobilis SYK-6 (2005), J. Bacteriol., 187, 5067-5074.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.57 expressed in Escherichia coli BL21(DE3) cells Sphingomonas paucimobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.57 EDTA complete inhibition at 0.5 mM, the oxygen consumption activity after EDTA treatment is completely recovered by addition of Fe2+ (119%) Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.57 0.0669
-
 gallate in 50 mM GTA buffer (pH 8.5) consisting of 50 mM 3,3-dimethylglutarate, 50 mM Tris, and 50 mM 2-amino-2-methyl-1,3-propanediol, at 30°C Sphingomonas paucimobilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.57 Fe2+ DesB requires Fe2+ for activity Sphingomonas paucimobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.57 45000
-
 2 * 45000, SDS-PAGE Sphingomonas paucimobilis
1.13.11.57 46843
-
 2 * 46843, calculated from amino acid sequence Sphingomonas paucimobilis
1.13.11.57 86000
-
 gel filtration Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.57 Sphingomonas paucimobilis Q5NTE5
-
-
1.13.11.57 Sphingomonas paucimobilis SYK-6 Q5NTE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.57 POROS polyethyleneimine column chromatography Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.57 gallate + O2
-
 Sphingomonas paucimobilis 4-oxalomesaconate
-
 ?
1.13.11.57 gallate + O2
-
 Sphingomonas paucimobilis SYK-6 4-oxalomesaconate
-
 ?
1.13.11.57 additional information no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate Sphingomonas paucimobilis ?
-
 ?
1.13.11.57 additional information no activity with protocatechuate, 3-O-methylgallate, gentisate, 2,3-dihydroxybiphenyl, 4-methylcatechol, 2,3-dihydroxybenzoate, and methylgallate Sphingomonas paucimobilis SYK-6 ?
-
 ?
1.13.11.57 pyrogallol + O2
-
 Sphingomonas paucimobilis ?
-
 ?
1.13.11.57 pyrogallol + O2
-
 Sphingomonas paucimobilis SYK-6 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.57 homodimer 2 * 45000, SDS-PAGE Sphingomonas paucimobilis
1.13.11.57 homodimer 2 * 46843, calculated from amino acid sequence Sphingomonas paucimobilis

Synonyms

EC Number Synonyms Comment Organism
1.13.11.57 DesB
-
 Sphingomonas paucimobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.57 30
-
-
 Sphingomonas paucimobilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.57 15 40
-
 Sphingomonas paucimobilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.57 8.5
-
-
 Sphingomonas paucimobilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.57 6 9
-
 Sphingomonas paucimobilis

General Information

EC Number General Information Comment Organism
1.13.11.57 physiological function the enzyme is involved in syringate catabolism, the pathway involving DesB plays an especially important role in the growth of Sphingomonas paucimobilis SYK-6 on syringate, and DesB is involved in gallate degradation Sphingomonas paucimobilis