EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | component A of NTA monooxygenase | the ability of component B of NTA monooxygenase to catalyze the oxidation of nitrilotriacetate to iminodiacetate and glyoxylate is completely dependent on the presence of component A | Aminobacter aminovorans | |
1.14.14.10 | FMN | NTA-Mo activity increases by a factor of 4 when 0.003 mM FMN is included in the assay mixture, there is 0.4 mol of FMN per mol of purified component B | Aminobacter aminovorans | |
1.14.14.10 | additional information | the activity is increased neither by additions of 20% (w/v) polyethylene glycol or sorbitol | Aminobacter aminovorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | 5,5'-dithiobis(2-nitrobenzoate) | 67% residual activity at 1 mM | Aminobacter aminovorans | |
1.14.14.10 | Ca2+ | 5% residual activity at 10 mM | Aminobacter aminovorans | |
1.14.14.10 | EDTA | 3% residual activity at 10 mM | Aminobacter aminovorans | |
1.14.14.10 | FAD | 24% residual activity at 0.1 mM | Aminobacter aminovorans | |
1.14.14.10 | NaCl | 54% residual activity at 250 mM | Aminobacter aminovorans | |
1.14.14.10 | p-hydroxymercuribenzoate | 12% residual activity at 1 mM | Aminobacter aminovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.42 | 0.0013 | - |
FMN | pH 7.8, 25°C | Aminobacter aminovorans | |
1.5.1.42 | 0.25 | - |
NADH | pH 7.8, 25°C | Aminobacter aminovorans | |
1.14.14.10 | 0.0013 | - |
FMN | in 40 mM TAPS buffer at pH 8.5 and 25°C | Aminobacter aminovorans | |
1.14.14.10 | 0.5 | - |
nitrilotriacetate | in 40 mM TAPS buffer at pH 8.5 and 25°C | Aminobacter aminovorans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | Co2+ | may replace Mg2+, enzyme activity is reduced to 80% of that with MgCl2 | Aminobacter aminovorans | |
1.14.14.10 | Mg2+ | required, 2 mM used in assay conditions | Aminobacter aminovorans | |
1.14.14.10 | Mn2+ | Mn2+ ions are able to replace Mg2+ but lead to a higher uncoupled NADH oxidation and enzyme activity is reduced to 70% of that with MgCl2 | Aminobacter aminovorans | |
1.14.14.10 | additional information | no nitrilotriacetate consumption is observed with Ca2+,Fe2+, Fe3+, Zn2+, Cu2+, or Ni2+ | Aminobacter aminovorans | |
1.14.14.10 | additional information | the enzyme contains less than 0.15 atom of Fe per mol of protein, indicating that neither Fe-sulfur clusters nor cytochromes are present | Aminobacter aminovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.10 | 36000 | - |
2 * 36000, component B of NTA monooxygenase, SDS-PAGE | Aminobacter aminovorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.42 | Aminobacter aminovorans | - |
i.e. Chelatobacter heintzii | - |
1.14.14.10 | Aminobacter aminovorans | - |
- |
- |
1.14.14.10 | Aminobacter aminovorans ATCC 29600 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.42 | - |
Aminobacter aminovorans |
1.14.14.10 | ammonium sulfate precipitation, phenyl Sepharose column chromatography, TMAE-Fractogel column chromatography, and phenyl Superose column chromatography | Aminobacter aminovorans |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.42 | -70°C, in presence of 2 mM dithiothreitol, stable over several months | Aminobacter aminovorans |
1.14.14.10 | -70°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol, at pH 7.8, several months, without any significant loss of activity | Aminobacter aminovorans |
1.14.14.10 | 4°C, crude extract, 50 mM Tris-HCl, 200 h, 50% loss of activity | Aminobacter aminovorans |
1.14.14.10 | 4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer at pH 7.8, 7 days, 70% loss of activity | Aminobacter aminovorans |
1.14.14.10 | 4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol and ammonium sulfate to 5% saturation, at pH 7.8, 7 days, 5% loss of activity | Aminobacter aminovorans |
1.14.14.10 | 4°C, purified component B of NTA monooxygenase in 20 mM HEPES buffer with 2 mM dithiothreitol, at pH 7.8, 7 days, 20% loss of activity | Aminobacter aminovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.42 | FMN + NADH + H+ | - |
Aminobacter aminovorans | FMNH2 + NAD+ | - |
? | |
1.5.1.42 | FMN + NADH + H+ | - |
Aminobacter aminovorans ATCC 29600 | FMNH2 + NAD+ | - |
? | |
1.14.14.10 | additional information | less than 2% reactivity with N-methyl-iminodiacetate, N-acetamido-iminodiacetate, N-hydroxyethyl-iminodiacetate, EDTA, trimethylamine, triethanolamine, dimethylamine, dimethylglycine, methylamine, sarcosine, anthranilate, betaine, N-methylglutamate, N-methylalanine, N-methylaspartate, N-acetylglutamate, N-methylphenylalanine, N-methylleucine, N-methyltryptophane, glutamate, aspartate, citrate, proline, imidazoleacetate, asparagine, and ethylenediamine-N,N'-diacetate | Aminobacter aminovorans | ? | - |
? | |
1.14.14.10 | additional information | less than 2% reactivity with N-methyl-iminodiacetate, N-acetamido-iminodiacetate, N-hydroxyethyl-iminodiacetate, EDTA, trimethylamine, triethanolamine, dimethylamine, dimethylglycine, methylamine, sarcosine, anthranilate, betaine, N-methylglutamate, N-methylalanine, N-methylaspartate, N-acetylglutamate, N-methylphenylalanine, N-methylleucine, N-methyltryptophane, glutamate, aspartate, citrate, proline, imidazoleacetate, asparagine, and ethylenediamine-N,N'-diacetate | Aminobacter aminovorans ATCC 29600 | ? | - |
? | |
1.14.14.10 | nitrilotriacetate + FMNH2 + H+ + O2 | specific substrate | Aminobacter aminovorans | iminodiacetate + glyoxylate + FMN + H2O | - |
? | |
1.14.14.10 | nitrilotriacetate + FMNH2 + H+ + O2 | specific substrate | Aminobacter aminovorans ATCC 29600 | iminodiacetate + glyoxylate + FMN + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.10 | homodimer | 2 * 36000, component B of NTA monooxygenase, SDS-PAGE | Aminobacter aminovorans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.10 | component B of NTA monooxygenase | - |
Aminobacter aminovorans |
1.14.14.10 | NTA monooxygenase | - |
Aminobacter aminovorans |
1.14.14.10 | NTA-MO | - |
Aminobacter aminovorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.42 | 25 | - |
assay at | Aminobacter aminovorans |
1.14.14.10 | 25 | - |
12% activity is left at 2°C, and no activity is observed at 40°C | Aminobacter aminovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.42 | 7.8 | - |
assay at | Aminobacter aminovorans |
1.14.14.10 | 7.8 | - |
pH optimum for NTA consumption in cell extracts. At pH 7.2 and 8.4, 25% of the maximum activity is obtained, and no activity is left at pH 6.0 or 9.0 | Aminobacter aminovorans |
1.14.14.10 | 8.5 | - |
pH optimum for the purified enzyme | Aminobacter aminovorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.14.10 | 7 | 9.6 | 35% activity of the purified enzyme remains at pH 7.0 and 9.6 | Aminobacter aminovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.10 | FMN | omission of FMN or replacement of FMN by FAD results in a residual activity of 2% when component B is not resaturated with FMN | Aminobacter aminovorans | |
1.14.14.10 | NADH | NADPH cannot replace NADH | Aminobacter aminovorans |