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Literature summary extracted from
- Functional analysis of fungal polyketide biosynthesis genes (2010), J. Antibiot., 63, 207-218.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.3.42 | expression in Aspergillus oryzae under the starch inducible alpha-amylase promoter, expression in Pichia pastoris | Alternaria solani |
| 5.5.1.20 | expression in Aspergillus oryzae under the starch inducible alpha-amylase promoter, expression in Pichia pastoris | Alternaria solani |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.42 | prosolanapyrone II + O2 | Alternaria solani | the enzyme is involved in solanapyrone biosynthesis | prosolanapyrone III + H2O2 | - |
? |  |
| 1.1.3.42 | prosolanapyrone II + O2 | Alternaria solani 548 | the enzyme is involved in solanapyrone biosynthesis | prosolanapyrone III + H2O2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.3.42 | Alternaria solani | D7UQ40 | - |
- |
| 1.1.3.42 | Alternaria solani 548 | D7UQ40 | - |
- |
| 5.5.1.20 | Alternaria solani | D7UQ40 | - |
- |
| 5.5.1.20 | Alternaria solani 548 | D7UQ40 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.3.42 | - |
Alternaria solani |
| 5.5.1.20 | - |
Alternaria solani |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.3.42 | prosolanapyrone II + O2 | the enzyme is involved in solanapyrone biosynthesis | Alternaria solani | prosolanapyrone III + H2O2 | - |
? | |
| 1.1.3.42 | prosolanapyrone II + O2 | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A | Alternaria solani | prosolanapyrone III + H2O2 | in an enzyme-catalyzed DielsAlder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | ? | |
| 1.1.3.42 | prosolanapyrone II + O2 | the enzyme is involved in solanapyrone biosynthesis | Alternaria solani 548 | prosolanapyrone III + H2O2 | - |
? | |
| 1.1.3.42 | prosolanapyrone II + O2 | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A | Alternaria solani 548 | prosolanapyrone III + H2O2 | in an enzyme-catalyzed DielsAlder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | ? | |
| 5.5.1.20 | prosolanapyrone III | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A. Prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | Alternaria solani | (-)-solanapyrone A | - |
? | |
| 5.5.1.20 | prosolanapyrone III | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A. Prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | Alternaria solani 548 | (-)-solanapyrone A | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.3.42 | Sol5 | - |
Alternaria solani |
| 1.1.3.42 | solanapyrone synthase | bifunctional enzyme: prosolanapyrone II oxidase/prosolanapyrone III cycloisomerase | Alternaria solani |
| 1.1.3.42 | SPS | - |
Alternaria solani |
| 5.5.1.20 | Sol5 | - |
Alternaria solani |
| 5.5.1.20 | solanapyrone synthase | bifunctional enzyme: prosolanapyrone II oxidase/prosolanapyrone III cycloisomerase | Alternaria solani |
| 5.5.1.20 | SPS | - |
Alternaria solani |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.42 | 30 | - |
assay at | Alternaria solani |
| 5.5.1.20 | 30 | - |
assay at | Alternaria solani |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.3.42 | 7 | - |
assay at | Alternaria solani |
| 5.5.1.20 | 7 | - |
assay at | Alternaria solani |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.3.42 | flavin | flavin-dependent oxidase, the enzyme possesses a flavin-binding domain | Alternaria solani | |
| 5.5.1.20 | flavin | flavin-dependent oxidase, the enzyme possesses a flavin-binding domain | Alternaria solani | |
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Release 2023.1 (January 2023)
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