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Literature summary extracted from

  • Lin, C.J.; Tseng, W.C.; Lin, T.H.; Liu, S.M.; Tzou, W.S.; Fang, T.Y.
    Characterization of a thermophilic L-rhamnose isomerase from Thermoanaerobacterium saccharolyticum NTOU1 (2010), J. Agric. Food Chem., 58, 10431-10436.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.3.1.14 food industry L-rhamnose isomerase is used in rare sugar production Thermoanaerobacterium saccharolyticum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Thermoanaerobacterium saccharolyticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 3.53
-
 D-ribose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 3.53
-
 L-rhamnose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 45.2
-
 L-Lyxose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 58.9
-
 L-Mannose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 121
-
 D-allose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Ca2+ the enzyme shows 1% activity in the presence of 1 mM Ca2+ as compared to 1 mM Co2+ Thermoanaerobacterium saccharolyticum
5.3.1.14 Co2+ 100% activity in the presence of 1 mM Co2+ Thermoanaerobacterium saccharolyticum
5.3.1.14 Mg2+ the enzyme shows 9% activity in the presence of 1 mM Mg2+ as compared to 1 mM Co2+ Thermoanaerobacterium saccharolyticum
5.3.1.14 Mn2+ the enzyme shows 61% activity in the presence of 1 mM Mn2+ as compared to 1 mM Co2+ Thermoanaerobacterium saccharolyticum
5.3.1.14 additional information the enzyme shows null activity in the absence of any ions, as well as in the presence of Cu2+ and K+ Thermoanaerobacterium saccharolyticum
5.3.1.14 Na+ the enzyme shows 19% activity in the presence of 1 mM Na+ as compared to 1 mM Co2+ Thermoanaerobacterium saccharolyticum
5.3.1.14 Zn2+ the enzyme shows 2% activity in the presence of 1 mM Zn2+ as compared to 1 mM Co2+ Thermoanaerobacterium saccharolyticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 48000
-
 x * 48000, SDS-PAGE Thermoanaerobacterium saccharolyticum
5.3.1.14 48960
-
 x * 48960, calculated from amino acid sequence Thermoanaerobacterium saccharolyticum

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Thermoanaerobacterium saccharolyticum D5LNE5
-
-
5.3.1.14 Thermoanaerobacterium saccharolyticum NTOU1 D5LNE5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 65.6
-
 crude extract, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 197
-
 after 3fold purification, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Thermoanaerobacterium saccharolyticum D-psicose
-
 r
5.3.1.14 D-allose
-
 Thermoanaerobacterium saccharolyticum NTOU1 D-psicose
-
 r
5.3.1.14 D-ribose
-
 Thermoanaerobacterium saccharolyticum D-ribulose
-
 ?
5.3.1.14 D-ribose
-
 Thermoanaerobacterium saccharolyticum NTOU1 D-ribulose
-
 ?
5.3.1.14 L-lyxose
-
 Thermoanaerobacterium saccharolyticum L-xylulose
-
 r
5.3.1.14 L-lyxose
-
 Thermoanaerobacterium saccharolyticum NTOU1 L-xylulose
-
 r
5.3.1.14 L-Mannose
-
 Thermoanaerobacterium saccharolyticum L-Fructose
-
 r
5.3.1.14 L-Mannose
-
 Thermoanaerobacterium saccharolyticum NTOU1 L-Fructose
-
 r
5.3.1.14 L-Rhamnose
-
 Thermoanaerobacterium saccharolyticum L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose
-
 Thermoanaerobacterium saccharolyticum NTOU1 L-Rhamnulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 ? x * 48000, SDS-PAGE Thermoanaerobacterium saccharolyticum
5.3.1.14 ? x * 48960, calculated from amino acid sequence Thermoanaerobacterium saccharolyticum

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
 Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 75
-
-
 Thermoanaerobacterium saccharolyticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 40 70 the activity is fully retained after 2 h incubation at 40-70°C Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 21.5
-
 D-ribose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 30
-
 L-Mannose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 33.9
-
 D-allose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 180
-
 L-rhamnose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 240
-
 L-Lyxose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 7
-
-
 Thermoanaerobacterium saccharolyticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.14 5 9 the enzyme is stable at pH values ranging from 5.0 to 9.0 Thermoanaerobacterium saccharolyticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.15
-
 D-ribose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 0.28
-
 D-allose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 0.51
-
 L-Mannose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 5.3
-
 L-Lyxose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum
5.3.1.14 50.8
-
 L-rhamnose at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 Thermoanaerobacterium saccharolyticum