EC Number | Application | Comment | Organism |
---|---|---|---|
5.3.1.14 | food industry | L-rhamnose isomerase is used in rare sugar production | Thermoanaerobacterium saccharolyticum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.14 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Thermoanaerobacterium saccharolyticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 3.53 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 3.53 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 45.2 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 58.9 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 121 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.14 | Ca2+ | the enzyme shows 1% activity in the presence of 1 mM Ca2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | Co2+ | 100% activity in the presence of 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | Mg2+ | the enzyme shows 9% activity in the presence of 1 mM Mg2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | Mn2+ | the enzyme shows 61% activity in the presence of 1 mM Mn2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | additional information | the enzyme shows null activity in the absence of any ions, as well as in the presence of Cu2+ and K+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | Na+ | the enzyme shows 19% activity in the presence of 1 mM Na+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | Zn2+ | the enzyme shows 2% activity in the presence of 1 mM Zn2+ as compared to 1 mM Co2+ | Thermoanaerobacterium saccharolyticum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 48000 | - |
x * 48000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
5.3.1.14 | 48960 | - |
x * 48960, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.14 | Thermoanaerobacterium saccharolyticum | D5LNE5 | - |
- |
5.3.1.14 | Thermoanaerobacterium saccharolyticum NTOU1 | D5LNE5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.14 | heat treatment, nucleic acid precipitation, and Q-Sepharose column chromatography | Thermoanaerobacterium saccharolyticum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 65.6 | - |
crude extract, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
5.3.1.14 | 197 | - |
after 3fold purification, at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.14 | D-allose | - |
Thermoanaerobacterium saccharolyticum | D-psicose | - |
r | |
5.3.1.14 | D-allose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | D-psicose | - |
r | |
5.3.1.14 | D-ribose | - |
Thermoanaerobacterium saccharolyticum | D-ribulose | - |
? | |
5.3.1.14 | D-ribose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | D-ribulose | - |
? | |
5.3.1.14 | L-lyxose | - |
Thermoanaerobacterium saccharolyticum | L-xylulose | - |
r | |
5.3.1.14 | L-lyxose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-xylulose | - |
r | |
5.3.1.14 | L-Mannose | - |
Thermoanaerobacterium saccharolyticum | L-Fructose | - |
r | |
5.3.1.14 | L-Mannose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-Fructose | - |
r | |
5.3.1.14 | L-Rhamnose | - |
Thermoanaerobacterium saccharolyticum | L-Rhamnulose | - |
r | |
5.3.1.14 | L-Rhamnose | - |
Thermoanaerobacterium saccharolyticum NTOU1 | L-Rhamnulose | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.14 | ? | x * 48000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
5.3.1.14 | ? | x * 48960, calculated from amino acid sequence | Thermoanaerobacterium saccharolyticum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.14 | L-RhI | - |
Thermoanaerobacterium saccharolyticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 75 | - |
- |
Thermoanaerobacterium saccharolyticum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 40 | 70 | the activity is fully retained after 2 h incubation at 40-70°C | Thermoanaerobacterium saccharolyticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 21.5 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 30 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 33.9 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 180 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 240 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 7 | - |
- |
Thermoanaerobacterium saccharolyticum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.14 | 5 | 9 | the enzyme is stable at pH values ranging from 5.0 to 9.0 | Thermoanaerobacterium saccharolyticum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.14 | 0.15 | - |
D-ribose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 0.28 | - |
D-allose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 0.51 | - |
L-Mannose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 5.3 | - |
L-Lyxose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum | |
5.3.1.14 | 50.8 | - |
L-rhamnose | at 65°C in 50 mM potassium phosphate buffer and 1 mM Co2+ at pH 7.0 | Thermoanaerobacterium saccharolyticum |